Screening and 16S rRNA Analysis of the Bacteria of Producing Chitin Deacetylase
Zhou Guo-ying He yuan-hao, Zhang Huai-yun Biotechnology Core Facilities Biotechnology Core Facilities Central South University of Forestry and Technology Central South University of Forestry and Technology Changsha, Hunan, China Changsha, Hunan, China Gyzhou2118@ Yuanhao_he@
Abstract—Chitin deacetylase (CDA) is an enzyme that catalyzes thermochemical alkaline deacetylation of crab chitin. To the hydrolysis of acetamine groups of N-acetyl-D-glucosamine in develop an alternative, controlled, nondegradative and well- chitin. Chitin deacetylase converting chitin to chitosan in fungal defined enzymatic process for chitosan production, a study of cell walls. A total of 208 strains was isolated from soil samples in fungal chitin deacetylases has been initiated. Chitin deacetylase the riverbank of Xiangjiang River and an island of Zhanjiang. 12 (CDA), is the enzyme that catalyzes the conversion of chitin to CDA producing strains was founded by color reaction that including three fungus, two bacterias and four actinomycetes. chitosan by the deacetylation of N-acety -D-glucosamine The highest CDA producing strain Z7 had been screened by residues, was first identified and partially purified from [8] enzyme activity assays. 16S gene of the strain was ampl
