Preliminary Study on apoptotic effect induced by N- butanol extract in Capparis spinosa L. on SGC-7901
Lei YU, Le-qiong XIE Yu-bin JI Institute of Materia Medica and Postdoctoral Programme Engineering Research Center of Natural Anticancer Drugs Harbin University merce Ministry of Education Harbin, China Harbin, China yulei912@ yulei912@
Abstract—The purpose of this article was to investigate the anti-tumor drugs and make full use of Capparis to apoptosis effect of the n-butanol extract from Capparis spinosa L. play its unique pharmacological activity, this study primarily (CSBE) on human Gastric Cancer cells SGC-7901. MTT assay explored apoptosis of SGC-7901 induced by CSBE through was used to measure the inhibitory rate of CSBE Morphological vitro experiment. changes of apoptosis were observed with fluorescence microscope. Flow cytometry was employed to analyse the apoptotic rate of CSBE on SGC-7901 cells. Laser confocal II. METHOD 2+ microscopy(LCM) was adopted to assess [Ca ]i in the cells. The proliferation of SGC-7901 cells were inhibited after treated with A. Determination of inhibition in SGC-7901 cells through CSBE in the dose of 5, 25, 50, 100, 200 and 400 μg/mL for 72 h, MTT assay and the IC50 was μg/mL. After SGC-7901 cells were The MTT assay was performed at first described by treated by CSBE for 40
