海南麒麟菜养殖群体遗传多样性的rapd分析.pdf

2009耳上海研究生学术论坛海南麒麟荣养殖群体遗传多样性的RAPD分析张婷上海海洋大学水产与生命学院,上海,201 306 摘要:主要用RAPD技术对中国海南地区6个麒麟菜养殖群体的遗传多样性进行了分析。从 30个引物中筛选出6个可扩增出多态性强,重复性好的引物,检测到43个位点,~3 kb之间,其中多态位点为42个,%。%%之间,Nei’S基因多样性指数Ⅳ 809— 之间,Shannon’S遗传多样性信息指数,~;~,~。MEGA4软件对6个群体UPGMA 聚类分析,6个群体中,S单独聚为一类,其他5个群体,LA与XC先聚在一起,然后与LD,L,NC 聚在一起。说明这6个群体间的遗传多样性高于群体内的;S群体的遗传多样性高于其他群体;6群体的藻体虽然从外形上有或无差别,但RAPD标记显示皆为同一个种。关键词:麒麟菜,RAPD,遗传多样性 Population ic Diversity ofEucheumatoid algae Cultivated inHainan Detected by RAPD Marker 08Marine Biology,ZHANG Ting Advisor:Professor SHEN He—ding,Professor HE Pei—min Abstract:In thispapeg Random Amplified Polymorphic DNA(RAPD)marker was used estimate the population ic diversity sixcultured populations ofEucheumatoid algae from Hainan provinces, primers were selected from 30primers and amplified 43reproducible and discernible and thefragments were from 300 bp to were 42polymorphic and themean percentage ofpolymorphic %.Thenumber(percentage) ofpolymorphic loci,Nei’S(1973)gene diversi谚(H),Shannon’S information index(I),ic distances and ic identity of6populations ofEucheumatoid were %%,-,- ,·,-,respectively,as revealed by POPGENE 32 software.