基因克隆过程.doc

生物化学课学号: 20115610 xxx 班级:临床医学五年某班姓名: 张某某基因克隆-1- 设计过程一、查到的关于 mPPAR alpha 的基因资料 1、 NCBI 里的资料 Mus musculus strain C57BL/6J chromosome 5 genomic contig, GRCm38 C57BL/6J MMCHR5_CTG5 LOCUS NT_039305 249181 bp DNA linear CON 23-FEB-2012 DEFINITION Mus musculus strain C57BL/6J chromosome 5 genomic contig, GRCm38 C57BL/6J MMCHR5_CTG5. ACCESSION NT_039305 REGION: 21039824..21289004 GPS_000874384 VERSION GI:372099023 DBLINK Project: 169 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 249181) AUTHORS Church,., Schneider,., Graves,T., Auger,K., Cunningham,F., Bouk,N., Chen,., Agarwala,R., McLaren,., Ritchie,., Albracht,D., Kremitzki,M., Rock,S., Kotkiewicz,H., Kremitzki,C., Wollam,A., Trani,L., Fulton,L., Fulton,R., Matthews,L., Whitehead,S., Chow,W., Torrance,J., Dunn,M., Harden,G., Threadgold,G., Wood,J., Collins,J., Heath,P., Griffiths,G., Pelan,S., Grafham,D., Eichler,., Weinstock,G., Mardis,., Wilson,., Howe,K., Flicek,P. and Hubbard,T. TITLE Modernizing reference genome assemblies JOURNAL PLoS Biol. 9 (7), E1001091 (2011) PUBMED 21750661 REFERENCE 2 (bases 1 to 249181) AUTHORS Church,., Goodstadt,L., Hillier,., Zody,., Goldstein,S., She,X., Bult,., Agarwala,R., Cherry,., io,M., Hlavina,W., Kapustin,Y., Meric,P., Maglott,D., Birtle,Z., 生物化学课学号: 20115610 xxx 班级:临床医学五年某班姓名: 张某某基因克隆-2- Marques,., Graves,T., Zhou,S., Teague,B., Potamousis,K., Churas,C., Place,M., Herschleb,J., Runnheim,R., Forrest,D., Amos-Landgraf,J., Schwartz,., Cheng,Z., Lindblad-Toh,K., Eichler,. and Ponting,. CONSRTM Mouse Genome Sequencing Consortium TITLE Lineage-specific biology revealed bya finished genome assembly of the mouse JOURNAL PLoS Biol. 7 (5), E1000112 (2009) PUBMED 19468303 REFERENCE 3 (sites) AUTHORS Lowe,. and E