活血通络汤对人脐静脉内皮细胞缺氧/复氧损伤CD54表达及中性粒细胞粘附率的影响 【摘要】 目的研究活血通络汤对人脐静脉内皮细胞(HUVEC)缺氧/复氧损伤CD54表达及中性粒细胞(PMN)粘附率的影响。方法制备兔含药血清;HUVEC复苏和培养后,建立缺氧/复氧损伤模型;将模型细胞分为5组,即正常对照组、模型组及活血通络汤高、中、低剂量组。上述分组处理后,倒置显微镜下观察其形态学变化,测定CD54表达及PMN粘附率。结果活血通络汤低、中、高剂量组能够抑制HUVEC CD54表达、降低PMN与HUVEC的粘附率,且作用呈剂量依赖性改变,其中活血通络汤中、高剂量组与模型组比较差异有统计学意义(P<)。结论活血通络汤能够抑制CD54的表达增多、改善血管内皮细胞在缺氧条件下的细胞粘附性,从而对血管内皮细胞起到保护作用,这可能是该方防治下肢深静脉血栓的作用机制之一。 【关键词】 活血通络汤;缺氧/复氧;血管内皮细胞;CD54;中性粒细胞;粘附率 Abstract:Objective To investigate the effect of Huoxuetongluo Decoction on expression of CD54 and polymorphonuclear adhesion during human umbilical vein endothelial cells (HUVEC) anoxia/ reoxygenation injury, and explore its possible mechanisms. Methods Rabbits serum containing Huoxuetongluo Decoction was prepared. Resuscitate and culture HUVEC, then establish the anoxia/ reoxygenation injury model of HUVEC. The model cells were divided into five groups:normal control, model group and group of high, medium, low dose of Huoxuetongluo Decoction. After dealt seperately, the morphologic change of cells were observed through the microscope, the expression of CD54 and the polymorphonuclear adhesion were determined. Results The group of low, medium and high dose of Huoxuetongluo Decoction inhibited the expression of CD54 and decreased the adhesion between polymorphonuclear and HUVEC, the effects were strengthened with increasing the dose of Huoxuetongluo Decoc
