川菊止痛胶囊对偏头痛模型大鼠的改善作用及机制研究.doc

川菊止痛胶囊对偏头痛模型大鼠的改善作用及机制研究
摘 要 目的:研究川菊止痛膠囊对偏头痛模型大鼠的改善作用及机制。方法:将SD大鼠随机分为正常组、模型组、化学药阳性对照组(佐米曲普坦片, 05 g/kg)、中药阳性对照rats were evaluated using the times of scratching head as indexes within 2 h after modeling (30 min as a period of time). 4 h after modeling, hemorheological parameters as whole blood viscosity (low-shearing, medium-shearing, high-shearing), plasma viscosity, erythrocyte aggregation indexes and erythrocyte rigidity indexes were determined by automatic hemorheology instrument. The levels of NO, NOS, ET-1, CGRP in serum and the levels of 5-HT, 5-HIAA, DA and NE in cerebral tissue were determined by ELISA. RESULTS: Compared with normal group, the times of scratching head was increased significantly in model group at different periods (P<). The serum levels of NO, NOS and CGRP were increased significantly (P<), while ET-1 level was decreased significantly (P<). The levels of 5-HT, DA and NE in cerebral tissue were decreased significantly (P<), while 5-HIAA level was increased significantly (P<). Whole blood viscosity (low-shearing), plasma viscosity, erythrocyte aggregation index and erythrocyte rigidity indexes were increased significantly (P<),plasma viscosity were decreased significantly (P<) . Compared with model group, the times of scratching head were decreased significantly in TCM positive control group (0-120 min), TCM positive control group (60-90 min), CZC high-dose (30-120 min) and medium-dose (60-90 min) groups (P< or P<). The whole blood viscosity (low-shearing) and erythrocyte aggregation indexes of TCM positive control group, CZC high-dose and medium-dose groups were decreased significantly (P< or P<); the serum levels of NO, NOS and CGRP in chemical drug positive control group and CZC high-dose and medium-dose groups were decreased significantly (P< or P<), while the serum level of ET-1 was increased significantly (P<); the levels of 5-HT, DA and NE were increased significantly in