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2022届贵阳护理职业学院学生毕业论文
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贵阳护理职业学院
2022届高职高专学生毕业论文
Noggin of BALP and OC of osteoblasts were determined by ELISA different time points (12h, 24h, 48h, 72h) after optimal dosage of noggin BMP2, IGF-I were added into the culture the expression level of BALP and OC were investigated by reverse transcription PCR at the same time.
Results: 1. The proliferation ability of MG63 cells treated with BMP2, noggin and IGF-I groups were significantly different compared with that of CON group(P<). The effects of three factors on MG63 cell was dose-dependently and time-dependently after cultured for 72h. The most effective growth of MG63 cells showed at 2×10-9 mol/L of BMP2 with the proliferation rate of ℅ and 1×10-8mol/L of IGF-1 with the rate of ℅, while 8×10-9mol/L noggin with the inhibitory proliferation rate of ℅, respectively.
2. The concentration of BALP treated with BMP2, noggin and IGF-I groups were significantly different compared with that of CON group (P<) for 48h, especially, the noggin group was extremely significant (P<), and the BALP mRNA expression were also significantly different (P<).
3. The concentration of OC treated with noggin groups were significantly different compared with that of CON group (P<) for 24h, but the OC mRNA expression were not significantly different (P>). The concentration of OC of BMP2 group were significantly different compared with that of CON group significantly different compared with that of CON group (P<) , and the OC mRNA expression were also significantly different (P<). The concentration of OC of IGF-I group were significantly different compared with that of CON grou
2022届贵阳护理职业学院学生毕业论文
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p significantly different compared with that of CON group (P<), and the OC mRNA expression were also significantly different (P<).
Conclusion: BMP2 and IGF-I promoted the proliferation of MG
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