: . 中国动物传染35），感染组小鼠经口感染 300 条旋毛虫肌幼虫，对照组口服等量 PBS。收集对照组和感染组小鼠在感染后第 35d 的盲肠内容物，提取 DNA，并对其进行 16S rRNA 测序及 生物信息学分析。结果发现，对照组小鼠和感染组小鼠肠道菌群的 Alpha 多样性没有显著差异，而 β 多样 性发生变化，表明两组的菌群结构不同。Metastats 分析发现，与 CK 组相比，旋毛虫感染 35 天后的小鼠 肠道中产丁酸的罗斯拜瑞氏菌（Roseburia）和抗炎及组织修复相关的梭菌属（Clostridium）丰度显著升高 （P<），而拟杆菌（Bacteroides）（P<）、乳酸杆菌（Lactobacillus）和 Ralstonia（P<）的丰 度显著下降。同样地，LEfSe 分析发现罗斯拜瑞氏菌的丰度明显升高（P<）。综上表明，肌幼虫期旋 毛虫感染小鼠肠道菌群的组成与对照组有明显的不同。。 关键词：旋毛虫；肌幼虫；肠道菌群；宿主寄生虫相互作用 Changes of Intestinal Microflora in Mice Infected with Trichinella Spiralis druing Muscle Larval Stage KANG Jie1*，TONG Ming-wei2，YANG Yong 2* ( and Technology Center of Fenyang College, Shanxi Medical University, Fenyang 032200，China；2. School of Basic Medical Science, Shanxi Medical University, Taiyuan 030000，China)
Abstract：In this study, the changes of the gut microbiota in mice induced by infection with Trichinella spiralis (T. spiralis) during the muscle larvae stage were investigated. Twelve SPF female BALB/c mice were randomly divided into the control group (CK) and infection group (Ts35). In the infection group, BALB/c mice were orally infected with 300 T. spiralis muscle larvae, while those in control group were given equivalent PBS only. Mice were sacrificed at 35
