刘天琪 江汉美 田宇 刘金敏 中圖分类号 文献标志码 A 1001-0408（2022）06-0729-06 DOI ， 4-vinyl-2-methoxyphenol. Fifty-eight compounds （relative percentage of %） were identified from C. chinensis stir-frying with wine， such as phenylethanol， β-caryophyllene， macrocarehe D. There were 24 common components in the three， and relative percentage of them were %， %， %， respectively. After processing， there were 49 new components， such as furfural， n-hexanoic acid， caryophyllene oxide. The results of PCA showed that the cumulative contribution rate of the former two principal components was 100%; comprehensive score of volatile components of C. chinensis was the highest， followed by C. chinensis stir-frying with wine and C. chinensis stir-frying with saltwater. CONCLUSIONS The quality of volatile components in C. chinensis is good; the volatile components in processed products are more than those in C. chinensis. KEYWORDS Cuscuta chinensis; Cuscuta chinensis stir-frying with wine; Cuscuta chinensis stir-frying with saltwater; volatile components; principal component analysis; headspace solid phase microextraction combined with gas chromatography-mass spectrometry 菟丝子为旋花科植物南方菟丝子Cuscuta australis R. chinensis ，味辛、甘，性平，归肝、肾、脾经，具有补益肝肾、固精缩尿、安胎、明目、止泻、消风祛斑之功效，用于临床治疗肝肾不足、腰膝酸软、阳痿遗精、遗尿尿频、肾虚胎漏、脾肾虚泻等[1]。现代研究表明，菟丝子含有挥发性类、黄酮类、甾体类和多糖类等成分，在治疗男性不育、糖尿病、卵巢早衰和抑制动脉粥样硬化等方面具有较好效果[2]。 2020年版《中国药典》（一部）收载的菟丝子炮制品为盐菟丝子[1]，此外其酒制品也较为常用[3]。菟丝子经炮制后，其有效成分及含量均发生变化，使得菟丝子的性味和疗效也发生改变[4-5]。由于菟丝子富含油脂，故挥发性成分是菟丝子的主要成分，具有一定的药理作用，如麦芽醇具有补肾固精、补益肝肾的功效，石竹烯可止痛，棕榈酸可抗肿瘤、降血脂[6]。目前，关于菟丝子的研究主要为黄酮类成分，对其挥发性成分研究不夠深入，如裴学军等[6]从5个不同产地15批菟丝子中共鉴定出52种挥发性成分，但分析不够全面。本研究通过使用食盐和黄酒两种常用辅料，分析炮制方法对菟丝子挥发性成分的影响。 固相微萃取（solid phase microextraction，SPME）技术作为一种新颖的样品前处理与富集技术，因操作简单、费用低廉，成为最常用的样品前处理方法之一[7]。顶空固相微萃取-气质联用（headspace solid phase microextraction combined with gas chromatography-mass spectrometry，HS-SPME-GC-MS）技术已被广泛用于医药行业的质量分析领域，常应用于不同药用部位、不同基原、不同产地中药材
