产前地塞米松对孕鼠和早产胎鼠IGF-I的影响.doc

作者简介:陈贵岭( 1970- ),女,山西医科大学第二医院副主任医师。主要研究方向:围生医学 1 产前地塞米松对孕鼠和早产胎鼠 IGF- Ⅰ的影响陈贵岭 1 成要平 2*( 1 山西医科大学第二医院医院妇产科 030001 ; 2* 山西医科大学第一临床医学院妇产科 030009 ,通讯作者) 【摘要】目的:地塞米松在促进早产胎鼠肺脏成熟的同时对孕鼠和早产胎鼠 IGF-I 的影响。方法:18 只孕鼠随机分为对照组(CT组) ,地塞米松单剂量组( SD- DEX ) ,地塞米松多剂量(MD- DEX ),在孕鼠怀孕 14、15天给予不同剂量的地塞米松,在孕鼠怀孕 天行剖宫产终止妊娠,电镜下观察胎鼠肺脏成熟度,放射免疫法测定孕鼠血清、胎鼠羊水中 IGF-I 的含量,进行同组间的相关性分析; 免疫组化法进行胎鼠肝脏和胎盘中 IGF-I 的表达, 进行同组间胎鼠肝脏与胎盘中IGF- Ⅰ的相关性分析。结果:产前地塞米松应用在促进早产胎鼠肺脏成熟的同时可以导致孕鼠血清中 IGF -Ⅰ含量明显下降,这与用药后孕鼠体重下降有相关性。孕鼠血清中 IGF -I和胎鼠羊水中 IGF- I没有相关性。结论:地塞米松在促进早产胎鼠肺成熟的同时影响了孕鼠血清和胎鼠羊水中 IGF -Ⅰ含量,影响孕鼠体重变化和胎鼠的生长发育。关键词:地塞米松,生长和胚胎发育,胰岛素样生长因子- I,大鼠,早产 Effect of dexamethasone on pregnant and preterm birth of fetal rat with IGF -I CHEN Guiling 1 CHENG YaoPing 2( 1 Second Clinical Medical college,Shanxi Medical University 030001 ; 2 Frist Clinical Medical College,Shanxi Medical University,030009 ; * CorresPonding author,Tel:********** E-mail:cglsummer@) [ Abstract ] Objective To explore the of dose of dexamethasone administered antenatally on lung morphogenesis of rat embroy and correlation with insulin-like growth factor-I(IGF-I) of pregnant rat and fetal amniotic. Methods Eighteen p regnant rats were randomly assigned to three group with 6 in each,control,single dose detamethasone (SD-DEM),multilate dose dexamethasone (MD-DEM).It injected dexamethasone of different dose on the 14 th,15 th .All fetuses were delivered by cesarean delivery on