白藜芦醇对胰腺癌细胞的抑制作用.doc

白藜芦醇对胰腺癌细胞的抑制作用.doc白藜芦醇对胰腺癌细胞的抑制作用
作者:金洲祥,李永国,朱少俊,王飞海
【摘要】目的检测白藜芦醇(resveratrol,Res)体外单独及联合化疗药物对胰腺癌MIAPaCa-2细胞的抑制作用。方法?摇在体外培养条件下观察Res,5-氟脲嘧啶(5-flurouracil,5-FU)和吉西他滨(Gemcitabine,Gem)分别对胰腺癌MIAPaCa-2细胞增殖的影响, 然后根据以上抑制结果,选择对MIAPaCa-2细胞抑制率在15%~30%的5-FU或Gem药物浓度,再与不同浓度的Res联合用药。在联合药物处理MIAPaCa-2细胞48 h后,同样用噻唑蓝(methyl thiazolyl tetrazolium,MTT)法检测联合用药,检测其对细胞增殖的抑制作用。结果?摇 Res,5-FU及Gem体外单独用药均能显著抑制MIAPaCa-2细胞的生长增殖, μmol/L 5- μmol/L Gem后,对MIAPaCa-2细胞增殖的抑制作用显著提高,与单独用药组比较,差异有统计学意义(P<)。结论体外Res能显著抑制人胰腺癌MIAPaCa-2细胞的增殖,Res联合5-FU或Gem能显著提高对MIAPaCa-2细胞增殖的抑制作用。
【关键词】白藜芦醇;胰腺肿瘤;增殖;5-氟脲嘧啶;吉西他滨
Inhibitory effect of resveratrol on the proliferation of MIAPaCa-2 cells in vitro
Abstact Objecive To explore the inhibitory effect of resveratrol(Res) alone or bined on chemotherapeutic agents, such as flurouracil(5-FU) or gemcitabine(Gem), on the cell proliferation of MIAPaCa-2 cells in vitro. Methods The cell groethyl thiazolyl tetrazolium(MTT) after treatment alone for 48 h. Based on the results above, the agent concentration ent bined ethod. parison of the inhibition rates betade. Results Single agent experiment sho alone had significant inhibitory effects on the cell proliferation of MIAPaCa-2 cells. The agent concentrations of 5-FU and Gem ol/L and μmol/L, respectively. Agent bination experiment shool/L 5-FU or μmol/L Gem had a remarkable increase in anti-proliferation effect on MIAPaCa-2 ce