钩藤多指标含量测定及化学计量学分析 夏曼玉.pdf

: .
中国药学杂志·min；
检测波长 327 nm（绿原酸）、 246 nm（异钩藤碱、钩藤碱、去氢毛钩藤碱、毛钩藤碱和喜果苷）； 柱温：35 ℃；
进样量：10 μL。结果 绿原酸、异钩藤碱、钩藤碱、去氢毛钩藤碱、毛钩藤碱和喜果苷分离良好，平均回
收率分别为 %、%、%、%、%、%。11 批钩藤上述成分的含量测定结果分
别为 ～、～、～、～、～、～
mg·g-1。化学计量学分析表明不同产地的钩藤质量存在一定差异。结论 建立的钩藤多指标含量测定方法
简单、准确，可为钩藤药材质量综合评价提供新的参考方法。
关键词：钩藤；多指标成分；含量测定；高效液相色谱法；化学计量学
中图分类号：R917,R282 文献标识码：A

Muti-components determination and chemometric analysis of Uncariae Ramulus Cum Uncis
XIA Man-yu1, ZHANG Liu-ji1,2*, TU Wan-qian2, LI Xiang-yang3, WANG Jian-xia4, WU Bing-fan1
（1. Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China; 2. Henan Academy of
Traditional Chinese medicine, Henan Engineering Center of Genuine Medicinal Materials Comprehensive
Development, Zhengzhou 450004, China; 3. Henan Institute for Food and Drug Control, Zhengzhou 450018,
China; 4. Henan Health Care College, Zhengzhou 450008, China）
ABSTRACT: OBJECTIVE To establish a method for simultaneous determination of chlorogenic acid ，
isorhychophylline, rhynchophylline, hirsuteine, hirsutine, and vincosamide in Uncariae Ramulus Cum Uncis, and to
evaluate its quality based on chemometric analysis. METHODS HPLC was used. The analysis was performed on
a Kinetex C18 column (100mm× mm, μm), with the mobile phase consisted of acetonitrile (A)-% phosphoric
acid water (containing g·L-1 1-buty-3-methylimidazolium chloride )(B) in gradient elution mode. The flow ratewas ·min-1, and the detection wavelength was