肉类掺假鉴别技术研究进展.doc

肉类掺假鉴别技术研究进展.doc肉类掺假鉴别技术研究进展
摘要:随着现代分析检测技术的发展,肉类掺假鉴别技术也得到了长足的发展,主要包括感官鉴定技术、酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)技术、聚合酶链式反应(polymerase chain reaction,PCR)技术及生物质谱技术等。在这些技术中,感官鉴定技术操作简单,但准确度还有待改进。ELISA技术已有商品化试剂盒,由于受蛋白活性的影响,使其应用范围受到限制。PCR技术具有检测灵敏度高、结果重复性好等优势,但操作繁琐耗时。生物质谱技术通过多肽鉴定肉类掺假成为了一个新的发展方向。本文综述了现阶段常见肉类成分的检测技术,并对各种肉类掺假鉴别技术进行总结与分析,期望为肉类食品安全监测提供理论参考。
中国 8/vie
关键词:肉类掺假;鉴别技术;感官鉴定技术;酶联免疫吸附;聚合酶链式反应;生物质谱
Abstract: S). Sensory-directed identification is easy to perform, but the accuracy needs to be improved. Many ELISA kits have already been mercially developed, but their application is limited due to protein activity. PCR has the advantages of high sensitivity and repeatable results despite being tedious and time consuming. The application of MS in peptide analysis has bee a neilk adulteration. This paper provides an overvieost mon techniques currently used to detect meat ingredients and the existing techniques for identifying meat adulteration are summarized and analyzed to provide a theoretical reference for meat safety monitoring.
Key eat adulteration; identification technology; sensory-directed identification; enzyme-linked immunosorbent assay (ELISA); polymerase chain reaction (PCR); mass spectrometry (MS)
DOI:-8123-201704010
中图分类号: 文献标志码:A :1001-8123(2017)04-0056-06
引文格式:
王守云, 袁明美, 封聪, 等. 肉类掺假鉴别技术研究进展[J]. 肉类研究, 2017, 31(4): 56-61. DOI:/