tuning antimicrobial properties of biomimetic nanopatterned surfaces martyna michalska资料.pdf

该【tuning antimicrobial properties of biomimetic nanopatterned surfaces martyna michalska资料 】是由【小舍儿】上传分享，文档一共【12】页，该文档可以免费在线阅读，需要了解更多关于【tuning antimicrobial properties of biomimetic nanopatterned surfaces martyna michalska资料 】的内容，可以使用淘豆网的站内搜索功能，选择自己适合的文档，以下文字是截取该文章内的部分文字，如需要获得完整电子版，请下载此文档到您的设备，方便您编辑和打印。:..NanoscaleViewArticleOnlinePAPERViewJournalTuningantimicrobialpropertiesofbiomimeticnanopatternedsurfaces?Citethis:DOI:,aFrancescaGambacorta,aRaluDivan,,c,dAndreySokolov,*aNaturehasamassedanimpressivearrayofstructuresthata?ordprotectionfrommicrobialcolonization/,controlledvariationofthefeaturesofmimeticsderivedfrometchedsiliconallowsfortuningoftheirantimicrobiale??ectiveagainstawiderangeofmicrobialspeciesandexceedtheperformanceofnaturalsurfaces;incontrast,materialswithshorter/blunternanopillars(<2μm)selectivelykilledspeci?,themechanismsbywhichbacteriaarekilledaredemonstrated,emphasizingthedependenceuponpillardensityandtipReceived16thJanuary2018,,real-[for,.,internalmedi-DOI:,implants(joint,dental,andcosmetic),foodpreparation,andtheagriculturalindustry].Introductionkeepingthesesurfacesfreeofmicrobes(anti-biofouling;).AmongthesenanotexturedNovelmaterialsarebeingusedincreasinglyinmedicalappli-materials,,patible10andalsoexhibitsbactericidalactivitywhilean-,.,cloggingSeveralmethodscanbeemployedtofabricatesuchnano-:16:(biofouling),–16Reactive-chingAdditionally,theemergenceofmulti-drug-resistantbacteria(RIE)isascalableandcost-e?ectivetechniqueduringwhichthatcannotbeeliminatedbytreatmentwithanantibioticorbSitopographicalfeaturescanbemodulatedbyvaryingpositionandflowrate,topreventand/ore?,etchingtime,pressure,DCself-biasvoltage,andRecently,–21thatexhibitantimicrobialpropertieshavegainedalotofatten-Tobeusedasane?ectiveantimicrobialagent,–includingblacksilicon(bSi),4patternedactonbacterialcellenvelopes,whichnaturallydisplayahightitaniumnanowires,5,6nanotexturedpolymers,–(.,cicada,dragonfly)urringwithnaturalnanotexturedsurfaceswherenanopillarsarethicker,blunt,denseandpromotebacterialadhesionbetweenmultipleaBiosciencesDivision,ArgonneNationalLaboratory,Argonne,IL60439,,essus-E-mail:******@,imposingstressonthebCenterforNanoscaleMaterials,ArgonneNationalLaboratory,Argonne,IL60439,outermembraneandcellwall,eventuallyreachingastretchingUSAthresholdthatcausesrupture,,ArgonneNationalLaboratory,Argonne,IL60439,USAdRecently,ur-DepartmentofBiomedicalEngineering,PennsylvaniaStateUniversity,UniversityPark,PA16802,USAringsurfaceshasbeenproposedwherecellsaredisruptedas?Electronicsupplementaryinformation(ESI):-?TheRoyalSocietyofChemistry2018Nanoscale:..ViewArticleOnlinePaperNanoscaleRegardless,nanotexturedsurface-mediatedcellkillingaMEGAPOSIT?SPR?220-(RohmdependsonthenatureofthecellenvelopeandvariesacrossandHaasElectronicMaterials,LLC),35sat3500rpm,,di?erencesinbactericidale?cacyofbakedfor2minat115°--andGram-positivebacteriahavebeenwidelyresistwasremovedbythreetimesacetone/,25–-texturedmaterial;po-,31Inthislineofreasoning,thecor-Scanningelectronmicroscopy(SEM)wasperformedusingarelationbetweentheantimicrobialpropertiesofvariousJEOL7500FieldEmissionScanningElectronMicroscope,naturalnanoprotrusionsfoundonthewingsofthreedi?erentoperatingat10–(P.),alterna--tively,,aneighbor-distances(NND)ImageJplugin(:YuxiongMao).Wenzelroughnesswascellsanddesignernanoprotrusionsiscruciallyneeded,withdeterminedasshowninESI(eqn(S1)–(2)).?theaimofengineeringsurfacesthatdisplayhigheranti-Staticwatercontactanglesweredeterminedusingadedi-,contact-angle-measurementdevice(FM40EasyDrop,challenges,bSiwithawiderangeofnanotopographieswasKRüSSGmbH,Hamburg,Germany).Here,5μldropletsofde-fabricatedusingtheRIEtechnique,andthebactericidale?ec-ionizedwaterwereplacedintothreedi?erentpositionsonthetivenessofthesematerialstowardsdiversebacterialgenerasamplesurfaces,andtheanglesofdropsweredeterminedand[Escherichia(E.),Pseudomonas,Rhodobacter(R.),andBacillusaveraged.(B.)]-ditionsreproduciblyalteredthefeaturesoftheseroughsur-(.,nanopillarlength,tipshape,andspacing)aswellasThreeGram-negativegeneraEscherichia,Pseudomonas,-positiveBacilluswereusedinthisstudyofbacteriawithbSisurfacesbyscanningelectronmicroscopy(TableS1?).-host-range(SEM)-(NCBI3610)werecul-Tothebestofourknowledge,thisisthefirstreportontheturedonLBmedium,aerobically,at37°?erencesinbSiantimicrobialper-(SBW25)wasgrownonLBmediumat28°:16:-positiveandGram-(ΔrshI[pBBRRWsLsM]35andspeciesbut,also–andpossiblymoreimportantly–amongU43[pBBR1MCS-2],respectively)(?1ofyeastextract,)andSRCVPY37medium,-aerobic,(125rpm,siliconespongeclosures,33°C).Whenappro-priate,gentamicin(24μgml?1)andkanamycin(30μgml?1)(4″,p-typeboron-doped,?100?,resistivity10–20Ωcm?1,525±25μm,)wereetchedbyRIEtechnique()attemperatureof20°-.,–materialinteractionsandtoalignwithanticipatedendprocess,-:RF100W,pressureThecultureswerethendilutedintofreshmediumtothecon-35mTorr,backcoolingelectrodeHe5Torr,×107cfuml?,both26m(entimeterperminute)m,--(asabenchmark)trallyinawell(Chryschem,HamptonResearch)-controlledexperimentalsystemtopreventevapor-1cm2materialssamples,full-sizewaferswerespin-coatedwithation().Toassureevenhumidity,1mlofsterilewaterNanoscaleThisjournalis?TheRoyalSocietyofChemistry2018:..ViewArticleOnlineNanoscalePaperwasaddedtoeachwell,thewafer/controlwasplacedontheSampleswerecriticalpoint-dried(CO2/EtOH),sputter-coatedtopofpedestal,20μlofcellswereadded,(22mmdia-meter).(RT,25±°C),cells(20μl)(DK400)38wasgrowninwereretrievedafterinsitudilution1:2withfreshmedium,TerrificBrothmediuminthepresenceofIPTG(100μM;Sigmaandseriallydilutedfurthertoobtain50–300coloniesperplateAldrich)at30°Cforapproximately14–～(OD600～2),colo-eitherabSi()orasmooth,,andbactericidale?ciencywasdeterminedTominimizeevaporation,the1cm2waferwasenclosedinabasedonthepercentageofbacteriarecoveredfrombSiversussealed,opticallyclearchamber(××).Toobservebacterialmotilityandinteractionofparisonofthecellswiththesiliconsurfaces,(10×objective,)andamonochromegrowthinstaticmicrofugetubes(evaluatedbyOD600measure-).Theexperimentswerecarriedoutinthesamemannerthroughmonochromatorswasat587and610nm,(videsupra).ThetestedcontrolsurfacesAframerateof15fps(66msexposures)wasfoundtobethewerenon-etchedsiliconwafersandglasscoverslides,andoptimumdataacquisitionspeedthatbalancesfluorescentsen-theirhydroxylatedvariantsgeneratedbyair-plasmaexposuresitivity,accuratetrackingofbacterialmotion,andreducedfor5min(fordetails,?).GlasscoverslideswerephotobleachingofGFP--eptablesignal-to-noiseratiosduringexperi-faceswasfoundinvarianttoairplasmatreatmentandmentsthatlasted20–-increasesinculturedensitymatchedthegrowthincultureterialdynamicsduringtheinitialbSiinteractionperiodtubes(measurementofopticaldensities;OD600).alloweddetectionofdistinctevents,.,---Whilethepositionofanindividualbacteriumwastrackedformedtovalidatetestsbasedontheplate-,theorientationwasdetectedautomaticallybasedonSimilarmethodswereusedbutwithhigherconcentrationofafastFouriertransformationofthebacteriumanditssur--×107cfuml?,thesurfaceswerewashedthreetimeswith1×TBStoremoveplanktonic(non-attached),followedbystainingwithLIVE/DEAD?BacLight?:16:(L7012,Invitrogen)perins