丹参酮ⅡA抑制非小细胞肺癌NCI-H520细胞生长的研究
简晓顺刘相富陈健清
[摘要] 目的: 探讨丹参酮ⅡA抑制非小细胞肺癌NCI-H520细胞生长及其分子机制。方法: 采用MTT法检测非小细胞肺癌NCI-H520细胞活力;采用流式细胞计检测非小细胞肺癌NCI-H520细胞周期;应用免疫印迹方法检测蛋白表达水平。结果: 丹参酮ⅡA显著抑制非小细胞肺癌NCI-H520细胞生长,并呈浓度依赖方式;流式细胞检测到丹参酮ⅡA作用非小细胞肺癌NCI-H520细胞,细胞周期S期细胞比例显著增多;免疫印迹实验检测到丹参酮ⅡA处理非小细胞肺癌NCI-H520细胞,细胞周期蛋白Cyclin E及CDK2抑制剂P27显著上调。结论: 丹参酮ⅡA可能通过上调P27抑制CDK2,导致非小细胞肺癌细胞NCI-H520细胞周期S阻滞。
[关键词] 丹参酮ⅡA;非小细胞肺癌细胞;P27;细胞周期
Tanshinone ⅡA induces renal cell carcinoma NCI-H520 cell cycle S-phase arrest by upregulation of CDK2 inhibitor JIAN Xiao-shun, LIU Xiang-fu, CHEN Jian-qin. Pharmaceutical Department, Guangzhou Medical University Cancer Institute and Hospital, Guangzhou 510095, China
[Abstract] Objective: To explore the molecular mechanism by which Tanshinone ⅡA (Tan ⅡA) inhibits non-small cell lung cancer NCI-H520 cells. Method: The viability of NCI-H520 cells treated with Tanshinone ⅡA was detected by MTT assay; Cell cycle profile of NCI-H520 cells induced by Tanshinone ⅡA was examined by Flow Cytometry; Immunoblotting was utilized to determine proteins expression level. Result: Tanshinone ⅡA remarkably detriments NCI-H520 cells viability in dose-dependent manner; cell cycle analysis indicated the proportion of NCI-H520 cells in cell cycle S-phase increased after treatment of Tanshinone ⅡA. Further, Immunoblotting showed the upregulation of cyclin E and CDK inhibitor P27, in NCI-H520 cells treated with Ta
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