肺气肿胶囊中的槲皮素和山柰素的含量测定.doc

肺气肿胶囊中的槲皮素和山柰素的含量测定
肺气肿胶囊中的槲皮素和山柰素的含量测定
作者:袁斌, 欧阳臻,缪亚东,江涛涛,沈绛英【关键词】肺气肿胶囊;高效液相色谱法;槲皮素;山柰素摘要:目的建立肺气肿胶囊中槲皮素和山柰素含量测定方法。方法采用高效液相色谱法,使用C18柱,流动相为甲醇-%磷酸溶液(60∶40),检测波长为370 nm, ml/min。~ 88~ μg范围内呈良好线性关系,%%,%%(n=5)结论该方法灵敏准确,重现性好,专属性强,可作为肺气肿胶囊的质量控制。关键词:肺气肿胶囊; 高效液相色谱法; 槲皮素; 山柰素 Determination of Quercetin and Kaempferol in Feiqizhong Capsules Abstract:ObjectiveTo establish a method to determine the content of quercetin and kaempferol in Feiqizhong was used to determine the content of quercetin and kaempferol with detection wavelength at 370nm. The mobile phase consists of a mixture of methanol-%H3PO4(60∶40). The flow rate was . ResultsThe linear regression for quercetin and kaempferol was obtained at the range of ~ and 88~ 6 μg. The average recovery of quercetin and kaempferol were % and %, RSD were % and % respectively(n=5). ConclusionThe method is sensitive, accurate, reproducible and exclusive. It can be used for quality control of Feiqizhong Capsules. Key words:Feiqizhong Capsules; HPLC; Quercetin; Kaempferol

肺气肿胶囊是由野马追等药材经过科学提取并加入适量辅料而制成的硬胶囊,具有补肾益气、活血化瘀、止咳祛痰的功效,主要用于治疗肺肾亏虚,血行不畅,痰浊阻肺所引起的胸闷憋气,动辄喘乏,咳嗽痰多,慢性气管炎,阻塞性肺气肿等证候。其治疗肺气肿的主要有效成分为黄酮类化合物,文献[1]报道黄酮类化合物具有降低血管脆性及异常通透性,能够扩张血管和降低血脂与胆固醇的作用,抑制血栓形成,同时还具有止咳、祛痰平喘的作用。为控制其质量采用测定水解苷类后的槲皮素和山柰素的含量。文献