蛋白质组研究中分离新技术与新方法.pdf

第 19 卷第 3 期生命科学 Vol. 19, No. 3
2007 年 6 月 Chinese Bulletin of Life Sciences Jun., 2007
文章编号: 1004-0374(2007)03-0281-08
蛋白质组研究中分离新技术与新方法
张维冰,张丽华,张玉奎*
(中国科学院大连化学物理研究所,大连 116023)
摘要:对于蛋白质组的研究离不开分析技术的支撑。由于样品及其基质的复杂性,为了实现蛋白质
的高通量、高灵敏度、快速分析鉴定,必须发展与之匹配的新技术与新方法。多维高效液相色谱/ 毛
细管电泳技术,部分弥补了传统 2D PAGE 的不足,近年来,在蛋白质分离鉴定方面取得了最令人瞩
目的成绩。本文分别从多维液相色谱分离技术、多维毛细管电泳蛋白质分离平台、微柱液相- 毛细管
电泳联用技术、极端 pH 蛋白质的分离分析和蛋白质的在线富集技术等方面对蛋白质组学研究中在新技
术与新方法方面近期取得的成果加以系统阐述。
关键词:蛋白质组;多维分离;高效液相色谱;毛细管电泳
中图分类号:Q503; Q816 文献标识码:A
New technology and method for proteomics research
ZHANG Weibing, ZHANG Lihua, ZHANG Yukui*
(Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China)
Abstract: There is indispensable analysis technology for proteomics research. Because of plexity of
sample and matrix, for high throughput, high sensitivity and rapid identification in proteomics research, we need
develop new technology and method to match this demand. Multidimensional HPLC/CE technology, partly
supply the limitation of traditional 2D PAGE. Recently, they gained notable achievements in protein separation
and identification. In this paper, prehensively elaborate the achievements from new technology and
method for proteomics research as follows: Multi-dimensional HPLC; Multi-dimensional CE; mHPLC-CE; ex-
treme PH protein analysis and on-line protein concentration tech