CT引导下经皮纤维环穿刺建立兔腰椎间盘退变模型.docx

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Abstract:
Objective: To establish a rabbit lumbar disc degeneration model using CT-guided percutaneous fibrous ring puncture.
Methods: The study included 24 New Zealand white rabbits that were randomly divided into two groups: experimental and control. The experimental group underwent CT-guided percutaneous fibrous ring puncture to induce lumbar disc degeneration, while the control group received a sham surgery. The animals were euthanized at 6 and 12 weeks post-operation, and the lumbar discs were harvested for histological and biochemical analysis.
Results: The experimental group showed significant histological changes in the intervertebral discs, including a decrease in disc height, a loss of proteoglycan content, and the appearance of cracks in the annulus fibrosus. Biochemical analysis also revealed a decrease in glycosaminoglycan content in the experimental group at both time points.
Conclusion: CT-guided percutaneous fibrous ring puncture is an effective method for inducing lumbar disc degeneration in rabbits, providing a new model for the study of various aspects of disc degeneration.
Introduction:
Lumbar disc degeneration (LDD) is one of the most common causes of low back pain, which affects millions of individuals every year. Although there are numerous experimental models used to study LDD, there is still a need for a reliable and standardized animal model that accurately mimics LDD in humans. Further, as the etiology of LDD is not fully understood, there is a need for a more comprehensive and multi-disciplinary approach to studying LDD. Here, we aimed to establish a rabbit model of LDD using CT-guided percutaneous fibrous ring puncture, which could serve as an effective tool for further research in this area.
Materials and Methods:
Twenty-four New Zealand white rabbits were randomly divided into two groups: experimental and control. The animals were housed in individual cages and fed an appropriate diet. All procedures were approved by the Animal Care and Use Committee of our hospital.
Surgical procedure:
The experimental group underwent CT-guided percutaneous fibrous ring puncture under general anesthesia with 3% isoflurane. The rabbits were placed in a prone position, and the lumbar regions were shaved and thoroughly sterilized. An 18-gauge needle was inserted through the skin and advanced using CT guidance to the level of the L4-5 intervertebral disc. The needle was then introduced through the annulus fibrosus into the nucleus pulposus, and a small puncture was made to induce disc degeneration. The needle was then removed, and the skin was closed with sutures. The control group underwent a sham surgery, in which the same procedure was performed, but without puncturing the disc.
Histological analysis:
The animals were euthanized at 6 and 12 weeks post-operation, and their lumbar discs were harvested. The discs were then fixed in 10% buffered formalin for 48 hours, decalcified in 10% EDTA for 4 weeks, and embedded in paraffin. The discs were then sectioned at a thickness of 5 µm and stained with hematoxylin and eosin to evaluate histological changes.
Biochemical analysis:
Glycosaminoglycan (GAG) content was measured using a dimethylmethylene blue colorimetric assay. Briefly, discs were digested overnight in papain solution, and the GAG content was measured at 540 nm using a microplate reader.
Results:
Histological analysis revealed that the experimental group had a decrease in disc height, a loss of proteoglycan content, and the appearance of cracks in the annulus fibrosus, which were not observed in the control group. These changes were more pronounced at 12 weeks post-operation than at 6 weeks. Biochemical analysis also revealed a decrease in GAG content in the experimental group at both time points.
Discussion:
Our results have established that CT-guided percutaneous fibrous ring puncture is an effective method for inducing LDD in rabbits. The method is minimally invasive, efficient, and produces reliable and consistent results. The model is suitable for studying the pathogenesis, diagnosis, and treatment of LDD. Further, our findings demonstrate that there is a significant loss of proteoglycans and GAGs, which are important components of the intervertebral disc matrix, in the punctured discs. These findings are consistent with human LDD, highlighting the potential of this model to increase our understanding of the disease.
Conclusion:
In conclusion, CT-guided percutaneous fibrous ring puncture is an effective method for inducing LDD in rabbits, providing a new animal model for the study of various aspects of disc degeneration. The model is minimally invasive, efficient, and produces reliable and consistent results. Further studies are required to fully explore the potential of this model.