SANTA投诉表(1).doc

COMPLAINT SHEET: WESTERN BLOTTING
Catalog #: sc-7938 Lot #: F0310 Order Date:
Catalog #: sc-7903 Lot #: H1710 Order Date:
A. Problem and Previous Experience
1. What is the specific problem you are experiencing?
When the degree of dilution was 1:500,there was no band. When the degree of dilution was 1:200,there was a dark background and no special band was assayed by western blot.
2. What size bands were expected and what size bands were detected?
The special band was assayed but there was no band.
3. Was the blot blank or was a dark background or non-specific bands seen?
When the degree of dilution was 1:500,there was no band. When the degree of dilution was 1:200,there was a dark background and no special band was assayed by western blot.
4. Did this same vial of product work in the past? What were the results?
Yes , the special band was assayed by western blot
5. Did other lots of this product work in the past? Which lots? What were the results?
Yes, Lot #:G0603. The special band was assayed.
B. Samples and Controls
1. From what species (animal) was the sample and what type of sample was used (cell lysate,tissue, purified protein, etc.)? What lysis buffer was used?
Normal rats.
Tissue.
Key Gen lysis buffer.
2. How much sample was loaded on the gel?
30 micrograms
3. If the blocking peptide was used, was it used as a negative control?