内蒙古荒漠生物土壤结皮中微生物多样性研究.docx

Microbial Diversity of Biological Soil Crusts from Inner Mongolia Deserts
Abstract
Biological soil crusts(BSCs) is of a special micronatural landscape plex mosaic of cyanobacteria, green algae, lichens, mosses, microfungi, and other bacteria in arid and semi-arid lands throughout the world. BSCs play an important role in desert ecosystems.
This essay is to reveal temporal-spatial scales variation of archaeal and fungi in BSCs
based on research of the diversity of these anisms in the desert of Mawusu and Hunshandade and could be an important supplement of research of the BSCs microbial diversity throughout the world.
This study constructed three archaeal clone libraries and three fungi clone libraries, corresponding to three samples, one was sampled in desert Maowusu in August 2009, others were sampled in desert Hunshandake in August 2009 and November 2008. The results showed these archaeal assemblages were consistently of very low ecological diversity, all of which were clearly related to Crenarchaeota. Through ic analysis, we got the same conclusion with foreign researchers. Fungal diversity was significantly higher than that of archaeal. These fungal belonged to two phyla, Basidiomycota and ycota. The vast majority of the sequences represented the ycota, which were divided into 13 orders of 7 classes, they were Hypocreales, Sordariales, coniochaetales, Eurotiales, Verrucariales, Chaetothyriales, Capnodiales, cryomyces, Dothideales, Pleosporales, Agyriales, Pezizales and Orbiliales. Basidiomycetes include Tremellales, Filobasidiales, Rhizoctonia, Malasseziaceae, Thelephorales and Aphyllophorales. ponent and dominant species of each fungi clone library were different, suggesting that the fungal diversity of biological soil crusts vary with the season and Location change. The situation is plex than that in North America and reflecting its special characteristics.
Key words: 16S rDNA; 18S rDNA; Archaea; Fungi; Phylogeny
Directed by: Associate Prof. Feng Fu Ying