高效液相色谱法测定沙苑子及其炮制品中沙苑子苷的含量.doc

高效液相色谱法测定沙苑子及其炮制品中沙苑子苷的含量
作者:贺成,唐晓晶,陈玉武,秦文杰,叶祖光
【摘要】目的建立沙苑子中沙苑子苷的含量测定方法,考察沙苑子炮制前后沙苑子苷的含量变化。方法采用高效液相色谱法,Apollo-C18( mm×250 mm,5 μm)色谱柱,以乙腈-%磷酸溶液(21∶79)为流动相, mL/min,检测波长为266 nm。 6~ 6 μg范围内线性关系良好(r= 9),%,RSD=%。结论本法简便、准确、重复性好、专属性强,为沙苑子药材质量控制提供了方法。盐炙可导致沙苑子中沙苑子苷含量下降。
【关键词】沙苑子;沙苑子苷;高效液相色谱法;含量测定

Abstract:Objective To establish a method for the determination planatuside in Semen planati, and study the content change planatuside processed. Method HPLC was used with Apollo-C18 column ( mm×250 mm, 5 μm), acetonitrile-%phosphoric acid (21∶79) as mobile phase, the flow rate at mL/min, and detected wavelength was set at 266 nm. planatuside showed a good linearity relationship in the range of 6~ 6 μg, r= 9. The average recovery was %, RSD was %. Conclusion The method is simple, accurate, reproducibility and strong specificity. It can be used for the quality control o
f Semen planati. Processing with salt can lead to content decrease planatuside in Semen planati.

Key words:Semen planatuside;HPLC;content determination

planatus ,主要分布于陕西、内蒙。味甘、性温,归肝、肾经,具有温补肝肾、固精、缩尿、明目等功效,用于肾虚腰痛、遗精早泄、白浊带下、小便余沥、眩晕目昏等病症[1]。近年来对其生药鉴别、药理活性及化学成分研究均有报道[2]。《中华人民共和国药典》2005年版收载沙苑子炮制方法为净制法(即除去杂质,洗净,干燥)、盐炙法(照每100 kg净药材用食盐2 kg的比例,将食盐用适量水溶解后,滤过,备用。取净沙苑子,用盐水拌匀。闷透,置锅内,以文火加热,炒干。炒至表面鼓起,色泽加深,呈深褐绿色或深灰褐色,取出,放凉即可)。