WARNING NOTICE: The experiments described in these materials are potentially hazardous and require a high level ofsafety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented. Legal Notices 4. Purification by Flash Column Chromatography . Competent Chemist Rating: “Looks Can Be Deceiving” Techniques Checklist: • Analyzing mixtures by TLC ❑ • Assembling a silica gel column ❑ • Applying crude mixtures to a silica gel column ❑ • Separating simple mixtures with a silica gel column ❑ Pre-lab Discussion and Required Reading: • Theory of column chromatography : Zubrick Ch. 27 • TLC - polarity/solvent systems: Zubrick Ch. 28, LLP Ch. • Setting up a silica gel column: Zubrick Ch. 29, LLP Ch. • Applying crude mixtures to the column • Running a flash column Equipment: • Flash Chromatography Column • Air flow apparatus (stopper, T-valve, screw clamp, tubing) • 100-mL Round-bottomed flask • Test tubes - 18x150 mm • Test tube rack • TLC plates and spotters • UV lamp • Large plastic funnels Digital Lab Techniques Manual: • 3. TLC: The Basics • 10.
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