rt-pcr(rt - pcr)
I. Experimental apparatus and materials:
1, transfer guns: 1ml, 200 L, 20 L, 10 L, 2 L
2, suction head: 1ml, 200 L, 20 L
3, homogenate tube: 5ml
4, suction head table: put 1ml suction head one, put 20 l suction head one
5, EP tubes: , , 100 L
6, reagent bottle: 2 60ml Brown reagent bottle (wide mouth, with cover)
1 125ml white reagent bottles (with absolute ethanol)
7, 50ml, 250ml, 500ml: a
8, capacity bottle: 250ml, 500ml, 1000ml
9, test tube rack: 5ml, , 20 L
10, salt water bottles: 250ml, 500ml each 2 spare, one with absolute ethanol, another with DEPC water
11, aluminum lunch box: 4
12, plastic small lunch box: 1
13, large porcelain cylinder: 2
14, tin paper: a roll
15 rolls of paper: 2 rolls
16, triangle flask: with a cap, slightly larger
Two, the processing and preparation of experimental equipment
1, plastic products: (including gun head, EP tube, homogenate tube, etc.)
The DEPC of water from the flask into the ceramic cylinder, the plastic products by soaking them, which requires a small tip Straw DEPC into the water, and then dried overnight, high pressure, spare, before the experiment will first put the gun suction head, and a high pressure (EP tube)
2, glass products: acid soaking overnight, washed clean, dry spare foil Mongolia (DEPC blister) (wash after the first bubble 1 per thousand DEPC overnight, and then dried)
3, homogenizer: (including scissors, tweezers) first wash, and then high pressure (do not need to bubble DEPC)
Three. Reagent preparation:
1, DEPC water: suck 1ml, placed in 1000ml double steamed water, with 1 per thousand DEPC water, placed in the 1000ml capacity bottle, static 4 hours standby.
2, 75% ethanol: with anhydrous ethanol DEPC water, and then put -20 degrees preservation (where DEPC water should be high pressure)
3, isopropyl alcohol: put brown bottle
4, chloroform: put in brown bottle
5 agarose
Four. Preparation of several buffers:
1 Electrophoresis buffer:
Tris 54G
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