依那西普对磨屑诱导骨溶解影响的实验研究.docx

依那西普对磨屑诱导骨溶解影响的实验研究作者:陈志荣,张亮,吴兴临,陆志东,金群华【摘要】[目的]检测依那西普(Etanercept,Enbrel)对钛颗粒刺激巨噬细胞(macrophages,MΦ)分泌肿瘤坏死因子α(tumornecrosisfactor-α,TNF-α)、白介素1(interleukin-1,IL-1)、白介素6(interleukin-6,IL-6)的影响,探讨依那西普防治人工关节无菌性松动的可能性。[方法]分离、培养小鼠腹腔巨噬细胞,2h后分为5组。A组:仅为MΦ;B组:MΦ+钛颗粒;C组:MΦ+钛颗粒+依那西普(10ng/ml);D组:MΦ+钛颗粒+依那西普(100ng/ml);E组:MΦ+钛颗粒+依那西普(1000ng/ml)。培养1h后,用酶联免疫法(ELISA)检测细胞培养上清液中TNF-α、IL-1、IL-6的含量。[结果]B组TNF-α、IL-1、IL-6含量明显高于A、D、E组(P)。[结论]钛颗粒可以刺激MΦ分泌大量的TNF-α、IL-1、IL-6,依那西普能够呈剂量依赖型地有效抑制钛颗粒诱导的MΦ分泌TNF-α、IL-1、IL-6,有望成为预防人工关节无菌性松动的药物。【关键词】依那西普;磨屑;骨溶解Abstract:[Objective]TodetecttheeffectofEtanerceptontheTiparticlesinducedTNF-αIL-1andIL-productionbymacrophageandtoevaluatethevalidityoftheetanerceptontreatmentofasepticlooseningofprosthesis.[Method],groupBwithMΦ+Tiparticles,groupCwithMΦ+Tiparticles+etanercept(10ng/ml),groupDwithMΦTiparticles+etanercept(100ng/ml),groupEwithMΦ+Tiparticles+etanercept(1000ng/ml).After1h,theproductionofTNF-αIL-1andIL-inculturesupernatantswasdetectedbyELISA.[Result]ThelevelsofTNF-αIL-1andIL-productionweremuchhigheringroupBthanthoseingroupA,D,E(P).[Conclusion]IsshowstheTiparticlescouldstimulateMΦtoexcreteprofuseTNF-ΑIL-1andIL-production,etanerceptcansignificantlyinhibitedtheproductionofTNF-αIL-1andIL-secretedbyTiparticlesinducedmacrophagesinadose-andidateforthepreventionofasepticloose