BSA-PLGA 缓释微球制备工艺的优化.doc

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实用医学杂志 2009 年第 25 卷第 15 期
·检验与临床·
BSA-PLGA 缓释微球制备工艺的优化
曾晗冰

李万里

徐华梓

李士
摘

要

目的: 以牛血清白蛋白(BSA) 为模型蛋白, 乳酸/ 羟乙酸共聚物(PLGA) 为载体, 优化微球的制备工
艺。方法: 采用复乳溶剂挥发法制备 BSA-PLGA 微球, 以包封率、载药量、粒径及 24 h 释放率等为观察指标, 通过
正交设计法优化显著影响微球制备工艺条件的 7 种因素。结果: 优化后的工艺条件是 BSA 用量为 5 mg、PEG 用量为
mL、PLGA 用量为 200 mg、PVA 浓度为 1%、NaCl 浓度为 10%、超声功率为 40 W、复乳搅拌速度为 1 000 r / min 。结
论: 该制备工艺简单、稳定, 可以得到包封率高、粒径适宜、突释较少的 BSA-PLGA 微球。
关键词
微球体;
血清白蛋白, 牛;
乳酸盐类;
聚合物;
药物载体
Optimization of preparation of BSA-PLGA controlled-release microspheres

ZENG Han-bing*,LI Wan-li,XU Hua-
zi, LI Shi. *Department of Spine Surgery, Second Affiliated Hospital, Wenzhou Medical College, Wenzhou 325027, China
Corresponding author :XU Hua-zi E-mail : spinexu@
【Abstract 】
Objective
To optimize the preparation technique for microspheres using BSA as model protein and
PLGA as controlled-release carrier. Methods
BSA-PLGA microspheres were prepared using (water-in-oil) in water
emulsion solvent evaporation technique. Using entrapment effciency , loading effciency , particle size , and 24 h-release
amount as the evaluating indicators , we optimized the 7 factors that markedly influenced the preparation technique for
microspheres by orthogonal factorization method. Results
The optimized parameters were BSA of 5 mg , PEG of
mL, PLGA of 200 mg , 1% PVA, 10% NaCl, ultrasonic power of 40 W , a