电刺激对大鼠脊髓损伤后神经生长因子表达的影响①.doc

中国康复理论与实践 2012 年 1 月第 18 卷第 1 期 Chin J Rehabil Theory Pract, Jan. 2012, Vol. 18,
DOI: .1006-
电刺激对大鼠脊髓损伤后神经生长因子表达的影响
陈虹 1a,李俊岑 1a,党彦丽 1a,刘姿辰 1a,杨拯 1b,张晓 1b

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·基础研究·
[摘要]

目的

探讨电刺激对成年大鼠脊髓损伤后脊髓灰质神经元神经生长因子(NGF)表达的影响。方法

健康成年 SD 大鼠
72 只,随机分为正常组、损伤对照组、电刺激组。采用 Allen 法,将后两组复制为脊髓 T9 损伤模型。术后对电刺激组大鼠进行电刺
激治疗 7 d。于术后 l d、3 d、5 d、7 d 对 3 组进行 BBB 评分,免疫组化和 Western blot 法检测 NGF 的表达情况。结果
BBB 评分结
果显示大鼠脊髓损伤后有自行恢复功能,从第 5 天开始电刺激组与损伤对照组有显著性差异(P<)。脊髓损伤后,电刺激组和损
伤对照组 NGF 表达均持续升高(P<),电刺激组表达多于损伤对照组(P<)。结论
从而创造了有利于神经再生的微环境。
脊髓损伤后电刺激诱导损伤区 NGF 表达,
[关键词]
脊髓损伤;神经生长因子;电刺激
Effects of Electrical Stimulation on Expression of Nerve Growth Factor in Adult Rats with Spinal Cord Injury

CHEN Hong, LI
Jun-cen , DANG Yan-li, et al. Chengdu Medical College, Chengdu 610083, Sichuan, China
Abstract: Objective To investigate the effects of electrical stimulation on the expression of nerve growth factor (NGF) in adult rats
with spinal cord injury. Methods 72 adult SD rats were randomly divided into 3 groups: damage control group, electrical stimulation group
and normal group. The spinal cord plete injury model on T9 was made with Allen's method. The electrical stimulation group received
electrical stimulation for 7 d. They were assessed with the Basso, Beattie & Bresnahan otor rating scale (BBB scale), and the expres-
sion of N