脊髓损伤大鼠运动及神经功能自然恢复规律的探讨.doc

脊髓损伤大鼠运动及神经功能自然恢复规律的探讨
王红星徐冬晨姚莉雷晓婷刘兴波丁新生王彤
【摘要】目的观察不完全脊髓损伤(SCI)大鼠运动及神经功能自然恢复情况,为SCI后运动训练时机选择提供依据。方法共选取45只成年SD大鼠,分为实验组(40只)和假手术组(5只)。实验组手术切除T10椎板暴露脊髓,采用改良Allens撞击法致SCI;假手术组仅手术切除T10椎板暴露脊髓。实验组分别于损伤前及损伤后1,3,5,7,14 d,21 d和28 d,假手术组分别于术前及术后1,3,5,7 d时采用斜板试验、改良Tarlov评分、BassoBeattieBresnahan(BBB)评分进行运动功能评定,采用脊髓诱发电位评定神经功能。实验组于上述各时间点分别取5只大鼠处死,假手术组于术后7 d时处死,取2组大鼠T10节段脊髓进行形态学检测。结果①实验组大鼠在损伤后1~3 d斜板角度、改良Tarlov评分和BBB评分均较损伤前显著降低,自损伤后5 d时开始增加,至14 d时达到平台期,显著高于术后1,3,5 d及7 d时水平(P<),与21,28 d时结果比较,差异无统计学意义(P>),但仍低于损伤前水平(P<)。假手术组术后与术前比较,差异均无统计学意义(P>)。②实验组大鼠在损伤后1 d时脊髓体感诱发电位(SCEP)潜伏期较损伤前明显延长(P<);随时间进展该潜伏期呈逐渐缩短趋势,至术后21 d时达到平台期,但仍显著长于损伤前水平(P>);波幅在损伤后1 d时明显降低,随时间进展呈逐渐增加趋势;假手术组术后各时间点潜伏期和波幅与术前比较,差异均无统计学意义(P>)。③2组大鼠术前脊髓结构完整,实验组术后1~3 d脊髓灰白质可见片状出血、细胞肿胀及变性;术后5~7 d炎性细胞减少,可见细胞内嗜碱性颗粒沉积、胶质细胞及少量神经纤维增生等;术后14~28 d可见胶质细胞、神经纤维增生明显,细胞内有空泡结构形成;假手术组大鼠脊髓形态学方面手术前后无明显改变。结论SCI大鼠运动功能、神经功能及脊髓病理形态学变化均与损伤时程密切相关,其运动功能改善一般于损伤后14 d时达到平台期,而神经功能改善一般于损伤后21 d时达到平台期。
【关键词】脊髓损伤;运动功能;脊髓诱发电位
Natural recovery of otor and neurological function in rats after spinal cord injuryWANG Hongxing*, XU Dongchen, YAO Li, LEI Xiaoting, LIU Xingbo, DING Xinsheng, WANG Tong. *Department of Rehabilitation Medicine, The 1st Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
Corresponding author: WANG Tong, Email: wangtong60621@
【Abstract】Objective To observe the natural recovery of otor and neurological functioning in rats after plete spinal cord injury. MethodsFortyfive SpragueDawley rats were divided into two groups: an experimental group and a shamoperation group. In the experimental group, the spinal cord was exposed at the T10 level by laminectomy, and an animal model of spinal cord injury (SCI) was created using a modification of Allens method. The shamoperation group received only laminectomy without spinal cord injury. otor and neurological functioning were evaluated using inclined plane tests, modified Tarlov scores, BassoBeattieBresnahan (BBB) scales and spinal cord e