,whichisabstractedindifferenttimestages()%sodiumchloridesolutionwhichcontains10%harose,+(Cacl2·6H2O),(H3BO3)inicebath,,:(1),itsloweddownandmaintainedacertainlevel,asforshatinyu,;Citrusgrandis,.(2),anensisbyLewis.(3)WemakeafurtherstudyonthereleasingbehavioroftheproteinfromPollenWallofshatinyuandCitrusgrandisbymicrosoft,,paredtheproteinabstractsolutionfrompollenwallofshatinyuwiththatofCitrusgrandisbythemeansofelectrophoresissuchasPAGE、SDS-PAGE、IEF-PAGE、IEF/SDS-:(1)binationwithCoomassiebrilliantblueR-250dyeing,Ⅴ,asforshatinyu,theproteinabstractsolutionfrompollenwallshowedmostofthetotalamountin5min,,60minor3hismoreorlessthanthatin40min.,ursinsegmentⅢ,Ⅳ,Ⅶ,andⅤ~Ⅵ.(2)paredwit
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