KirstenBomblies,adaptedfromFrançoisParcy'sprotocolWithalterationsspecificforfrankslab13March2007Harvesttissueandplaceincold90%,,butthesehavetobepolystyrene,,thenfixatroomtemperaturefor20--Gluc(seebelowforrecipe),andaddstainingbufferwithoutX--glucinanewtubetoafinalconcentrationof2mM-froma100mMstocksolutionofX-GlucinDMF-thismustbekeptinthedarkat-20°-gluc(washbuffer)fromsamplesandaddstainingbufferwithX-,onice,'t,°C(Iusuallydoitovernight,(overtwodays)%,35%and50%(recipebelow)for30minu
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