Abstract Objective:pare the fixation effects of nerve fibers by three fixatives, 10% formaldehyde, acetone, and bouin so as to provide experimental proof for making rapid frozen section of nerve fibers clinically. Methods: Sciatic nerves dissected from healthy rat were subjected to the technique of frozen section and then followed by fixation, and HE staining. The tissue structure of each HE-stained sciatic nerve frozen section was carefully examined. Results: Sciatic nerve frozen section fixed by 10% formaldehyde exhibits relatively clear tissue structure but undergoes tissue structure shrinkage; frozen section fixed by acetone exhibits good staining effect with no tissue structure shrinkage observed; frozen section fixed by bouin exhibits both clear tissue structure and good staining effect yet with the most tissue structure shrinkage. Conclusion: The three fixing agents evaluated here could all be used to fix nerve fiber while apparently acetone provides the best fixing effect.
Key words Fixative; Frozen section; HE staining 冰冻切片是病理科最常用的快速制片方法之一,它是借助低温使组织达到一定的硬度然后切片的一种
