HER4 在涎腺黏液表皮样癌中的表达及临床病理相关性.doc

cErbB4/HER4 在涎腺黏液表皮样癌中的表达及临床病理相关性
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作者:杨邵东曾鸣,刘德裕2,谢福生
【摘要】目的:探讨黏液表皮样癌(mucoepidermoid carcinoma,MEC)中表皮生长因子受体4(human epidermal growth factor receptor 4, HER4)的表达及与MEC临床病理因素的关系。方法:采用SP免疫组化方法检测45例黏液表皮样癌和15例正常涎腺组织中HER4的表达。结果:HER4表达主要定位于肿瘤细胞的胞浆,当着色强时,通常可见完整或不完整的胞膜着色。MEC阳性表达31例(69%),阴性表达14例(31%)。HER4的表达与性别、年龄、部位、肿瘤直径、临床症状、淋巴结转移、临床分期无关(),而与组织学分级呈明显负相关()。结论:HER4的表达与MEC组织学分级呈负相关; ErbB4癌基因可能参与MEC的发生与发展,需积累更多的资料研究,以明确其生物学作用。
【关键词】涎腺; 黏液表皮样癌;表皮生长因子受体4;免疫组织化学法

[ABSTRACT] Objective: To assess the expression of HER4 in mucoepidermoid carcinoma of salivary gland origin, and its correlation to clinicopathologic factors of mucoepidermoid carcinoma. Methods: A total of 45 cases of mucoepidermoi
d carcinoma and 15 normal salivary gland tissues were examined using a standard streptavidinbiotin method. Results: Immunostaining was observed predominantly in the cytoplasm. When cytoplasmic staining was strong, a partial plete circumferential membrane pattern of immunostaining could be seen. A total of 31 cases (69%) showed positivity and 14 cases (31%) were negative. There was no association between the expression of HER4 with age, sex, tumor site, tumor size, nodal metastasis, and clinical stage (), but significant adverse association between the expression of HER4 with histological grade was noted (). Conclusions:HER4 receptor protein has high negative correlation with histological grade in MEC, and maybe play a certain role in initiatation and developing of salivary MEC. Further studies are required to elucidate the biological behavior of HER4 in MEC.

[KEY WORDS] Salivary gland; Mucoepidermoid carcinoma; HER4; Immunohistochemistry
人第四表皮生长因子受体(Human epidermal grow
th factor receptor 4, HER4)由cErbB4基因编码,通过自身磷酸化参与细胞的信号传递,最终调节细胞的生长及分裂。近来研究表明, HER4介导的信号通路与多种肿瘤发生、发展及转移相关[1]。目前在涎腺肿瘤中尚无HER4的研究报道。黏液表皮样癌(mucoepidermoid carcinoma,MEC)是最常见的涎腺恶性肿瘤之一,约占涎腺上皮性恶性肿瘤的30% ,不同组织学分级的MEC预后相差显著且高度恶性MEC易转移,严重危害生命[2]。本研究拟检测HER4在涎