花生根中白藜芦醇分离纯化研究.pdf.pdf

Oct. 2008 现代化工第 28 卷增刊(2)
· 362 · Modern Chemical Industry 2008 年 10 月
花生根中白藜芦醇分离纯化研究
张初署,禹山林,潘丽娟,杨庆利
(山东省农科院花生研究所,山东青岛 266100)
摘要:研究了花生根中白藜芦醇的分离纯化。通过静态吸附解吸实验选出 DM- 130 大孔树脂,并确定其最佳吸附条件为:
上柱液流速为 015 mL/ min ,白藜芦纯质量浓度为 125μg/ mL ;最佳洗脱条件为:洗脱剂为 60 %( 质量分数) 的乙醇溶液, 洗脱流速
为 115 mL/ min ,洗脱液经浓缩、冷冻干燥后,用高效液相色谱测其纯度为 3017 %。
关键词:白藜芦醇;大孔吸附树脂;分离纯化;花生根
中图分类号:S56512 文献标识码:A 文章编号:0253 - 4320 (2008) S2 - 0362 - 04
Study on separation and purification of resvera trol from peanut root
ZHANG Chu2shu , YU Shan2lin , PAN Li2juan , YANG Qing2li
( Shandong Peanut Research Institue , Shandong Academy of Agricultural Sciences , Qingdao 266100 , China)
Abstra ct : The separation and purification of resveratrol from peanut root were studied. The macroporous adsorption resin
DM2130 were selected from 10 kinds of carriers by static adsorption and desorption test . The optimum adsorption conditions were
asfollows: the feeding rate was 015 mL/ min , the mass concentration of resveratrol was 125 μg/ mL ,the optimum elution
conditions were 60 % ethanol as eluent ,the elution rate at 115 mL/ min. The products was concentrated and freeze2drying ,the
mass content of resveratrol was 3017 % by HPLC.
Key wor ds: resveratrol ; macroporous adsorption resin; separation and purification ; peanut root
白藜芦醇(又名芪三酚) ,是含有芪类结构的非
1 材料与方法
黄酮多酚类化合物,在抑制癌细胞、降低血脂、防治
心血管疾病、抗氧化、延缓衰老等方面有较显著的作 111