初发鼻咽癌与复发鼻咽癌差异表达基因的研究.doc

初发鼻咽癌与复发鼻咽癌差异表达基因的研究
作者:黄振校,李文峰,杜季梅,黄亚,谭映霞,方渭清,廖志苏
【摘要】目的:通过全基因组基因芯片研究初发鼻咽癌(pNPC)与复发鼻咽癌(rNPC)之间的差异表达基因。方法:选择rNPC和pNPC组织标本,应用Affymetrix ST芯片,构建表达谱,检测两者之间的差异表达基因。结果:rNPC组与pNPC组比对,发现差异基因46个,下调基因38个,上调基因8个。这些基因参与钙结合、蛋白结合、酶活性调节等过程。结论:rNPC和pNPC之间存在基因差异表达,为了解rNPC的发生机制的研究起了一定的作用。
【关键词】复发;鼻咽肿瘤;基因芯片;基因表达
Abstract: Objective: To study the differentially expressed genes between relapsed nasopharyn-geal carcinoma (rNPC) and primary nasopharyngeal carcinoma(pNPC)by the whole human genome gene chips. Methods: pNPC tissue samples and rNPC tissue samples were selected,and Affymetrix ST chips were used to construct the expression profiling of each tissue sample. The differentially expressed genes were examined with bioinformatics. Results: A total of 46 genes was identified to be differential expressed in rNPC. Thirty eight genes were down-regulation,8 genes were up-regulation. These genes participated in the processes of calcium ion binding,protein binding,adjustment of enzyme acti
vity genes and so on. Conclusion: The differentially expressed genes exist between pNPC and rNPC,it may play an important role in the mechanism of occurrence and development of rNPC.
Key words: relapsed;nasopharyngeal neoplasma;gene chips;gene exprssion
鼻咽癌是头颈部常见的鳞状细胞癌之一。它具有明显的区域分布特征:在亚洲人中常见,特别是中国南方和东南亚地区(患病率:15~50/100 000)[1]。目前,鼻咽癌的发生机制尚未明确。它的发病可能与环境因素、饮食,特别是EB病毒感染等因素有关[1-2]。近些年研究发现,大量的