核酸提取试剂盒说明书.pdf

SFoeucortnhdEEdditiitoionnDecember201045QIAamp®ViralRNAMiniHandbookForpurificationofviralRNAfromplasma,serum,cell-freebodyfluids,andcell-culturesupernatantsSample&AssayTechnologiesQIAGENSampleandAssayTechnologiesQIAGENistheleadingproviderofinnovativesampleandassaytechnologies,,high-:IPurificationofDNA,RNA,,visittentsKitContents4Storage4IntendedUse5SafetyInformation5QualityControl5Introduction6Principle6Procedure6CellularDNAcontamination8Warningsandprecautions9Samplevolumes9Lysis9CarrierRNA10Additionofinternalcontrols10Spinandvacuumprocedures11AutomatedviralRNApurificationontheQIAcube11Determinationofyield11DeterminationofviralRNAlength11EquipmentandReagentstoBeSuppliedbyUser13ImportantNotes14Preparationofreagents14HandlingofQIAampMinicolumns17HandlingguidelinesfortheQIAvac24Plus18Centrifugation21ProtocolsPurificationofViralRNA(SpinProtocol)22PurificationofViralRNA(VacuumProtocol)25SampleConcentration29PurificationofCellular,Bacterial,orViralDNAfromUrine30TroubleshootingGuide31Appendix35OrderingInformation37QIAampViralRNAMiniHandbook12/20143KitContentsQIAampViralRNAKit(50)(250)(2ml)2001000BufferAVL*31ml5x31mlBufferAW1*(concentrate)19ml98mlBufferAW2†(concentrate)13ml66mlBufferAVE†3x2ml10x2mlCarrierRNA(polyA)310µg5x310µgSelectionGuide11*.†(15–25°C);