胰岛素治疗局灶脑缺血再灌注损伤的实验研究.doc

胰岛素治疗局灶脑缺血再灌注 损伤的实验研究        【摘要】目的:观察胰岛素对脑缺血再灌注损伤的治疗作用并探讨其作用机制。方法:制备易卒中型肾血管性高血压大鼠(RHRSP),用线栓法复制大脑中动脉阻塞(MCAO)模型。实验一:造成缺血6h再灌注18h,治疗组于不同时程使用胰岛素,测定脑梗死体积及脑水肿的变化。实验二:造成缺血2h再灌1、3、5天,治疗组于再灌注后立即使用胰岛素,采用TUNEL法原位标记DNA片段,检测TUNEL阳性细胞的变化。结果:治疗组的梗死灶体积及其占全大脑体积百分比、两半球体积差值显著减小,TUNEL阳性细胞亦明显减少(P<)。结论:胰岛素能减轻脑缺血再灌注损伤,早期用药效果更好,减少再灌注后细胞损伤很可能是其作用机制之一。【关键词】胰岛素高血压脑缺血再灌注损伤大鼠TheexperimentalstudyonreperfusioninjuryaftercerebralischemiatreatedwithinsulinHuangHaiwei,SuQingjie,HuangRuxun,etal.(DepartmentofNeurology,TheFirstAffiliatedHospital,SunYat-) 【 Abstract】 Objective::lusion(MCAO)instroke-pronerenovascularhypertensionrats(RHRSP)-mediateddUTP-biotinnickendlabelingDNAfragmentsinsitu(TUNEL),theirreversiblydamagedneuronsweredetectedat1、3、:betweenbothhcmispherevolumeweresignificantlylowerinthegroupwithtreatedimmediatelyafterMCAOandreperfusionthanthegroupswithouttreated(P<).ThenumbersoftheTUNELpositiveneuronswerealsosignificantlylowerinthetreatedgroupsthancontrolgroups(P<).Conclusions:Insulinmayhavetheeffectsinreducingrepe