RNA干扰技术的应用马欣荣Transgenicgenesilencing高通量的研究基因功能基因敲除geneknockout,herapeutics基因表达调控一、基因功能研究RNAihasbeapowerfultoolwithwhichtodissectgenefunctioninplant,,Drosophila,icinterferencebydouble-strandedRNAinArabidopsisthaliana,Chiou-,PNAS,April25,2000,,no9,4985-4990RNAi技术研究了拟南芥的AGAMOUS(AG),CLAVATA3(CLV3),APETALA1(AP1),PERIANTHIA(PAN)四个开花相关基因Constructvectors(pCGN1547):Gene-specificsequencesinthesense,antisenseorientationsTransformArabidopsisproduceduplexformationRNAsRNAinterferenceFlowersofwild-type,ag-1andAG(RNAi)plantsPhenotypesofwild-type,CLV3(RNAi),andclv3-2plantsPhenotypesofwild-type,ap1andAP1(RNAi)flowers
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