深圳扁穗牛鞭草种质遗传多样性的ISSR 分析[J].doc

扁穗牛鞭草种质遗传多样性的ISSR分析
范彦1,2,李芳1,张新全1* ,马啸1
(1. 重庆市畜牧科学研究院重庆 400039;2 四川农业大学草业科学系,四川雅安 625014.)
摘要:用ISSR标记对来自中国西南地区(四川、重庆、贵州)的28份扁穗牛鞭草材料的遗传多样性进行了检测。从96个ISSR引物中共筛选出13个多态性明显、反应稳定的引物,对28份材料DNA共扩增出129条谱带,,%。~,表现出丰富的遗传多样性。通过聚类分析和主成分分析,将28份扁穗牛鞭草分为两大类,同一地区的扁穗牛鞭草品种(系)基本聚在同一类,呈现出一定的地域性分布规律。基金项目:重庆市自然科学基金(CSTC,2006BA1022);教育部“新世纪优秀人才支持计划”(NCET-04-0909)。
作者简介:范彦(1973-),男,四川乐山人,四川农业大学在读博士,研究方向:牧草与草坪草种质资源及育种。E-mail:cq_fy001@;Tel:023-46792513
关键词:扁穗牛鞭草;种质;ISSR;遗传多样性分析
ic Diversity of pressa Germplasm Detected by Inter-simple Sequence Repeat (ISSR)
FAN Yan1,2, LI Fang1,ZHANG Xin-Quan1*, MA Xiao1
(1. Department of Grassland Science, Sichuan Agricultural University, Sichuan Ya’an 625014,China)(2. Chongqing Municipal Institute of Animal Husbandry Chongqing 400039, China)
Abstract: Inter-simple sequence repeat (ISSR) method was applied to detect ic variation of 28 pressa accessions from Southwest China (Sichuan Province, Chongqing City and Guizhou Province). Thirteen primers were selected from 96 ISSR primers, and 129 DNA fragments were amplified from 28 samples. Of which, 110 fragments were polymorphic (percentage of polymorphic bands was %), and the average number of DNA bands was per primer. The ic similarity among all accessions ranged from to , which indicated that the ic diversity paratively rich among the tested accessi