血清指标测定试剂盒说明书汇总.pptx

(BUN)测试盒说明书-脲酶法leafletPart1谷丙转氨酶(ALT/GPT)测试盒说明书-赖氏法Part2谷草转氨酶(AST/GOT)测试盒说明书-赖氏法Part3尿酸测试盒说明书-50T/48样Part4丙二醛(MDA)测试盒说明书-硫代巴比妥胺法Part5总超氧化物歧化酶(T-SOD)测试盒说明书-羟胺法Part6溶菌酶检测试剂盒说明书-比浊法Part7目录CONTENTS胆红素测试盒说明书-测总胆红素、一分钟胆红素Part8总抗氧化能力(T-AOC)测定试剂盒Part9白蛋白测定试剂盒-溴甲酚绿比色法Part10谷胱甘肽过氧化物酶(GSH-Px)测试盒Part11肌酐测试盒说明书-除蛋白苦味酸比色法Part12碱性磷酸酶(AKP)测试盒说明书-微量酶标法Part13蛋白定量测试盒说明书-考马斯亮蓝法(CBB)Part14Part15葡萄糖测定试剂盒(葡萄糖氧化酶-过氧化酶法)目录CONTENTSRLeafletofureanitrogentestbox-ransaminase(ALT)/glutamic-pyruvictransaminase(GPT)testbox-Reitman-FrankmethodmethodPart2Instructionbookofaspartate天门冬氨酸aminotransferase(AST)/Glutamicoxalacetic草酰乙酸transaminasetestbox-Reitman-FrankmethodPart3Specificationofuricacid(UA)testbox-50T/48simplePart4Leafletofmalonaldehyde(MDA)testbox-Glucosinolates芥子油苷、硫配糖体barbitoneAmine(TBA)methodPart5Mannualoftotalsuperoxidedismutasetestbox–hydroxylaminemethodPart6Instrucitonbookoflysozymetestbox-TurbidimetrymethodPart7目录CONTENTSSpecificationofbilirubin胆红素testbox-Part8LeafletofantioxidativecapacitytestboxPart9Mannualofalbumintestbox-bromocresolcolorimetryPart10InstructionbookofglutathioneperoxidasetestboxPart11Specificationofcreatinine肌酐testbox-deproteinization脱蛋白作用picricacidcolorimetryPart12Leafletofalkinephosphatase(AKP)testbox-traceenzymeCalibrationmethod(微量酶标法)Part13Mannualofproteinquantificationtestbox-coomassiebrilliantblue(CBB)methodPart14Part15Instructionbookofglucosetestbox(glucoseoxidase-peroxidasemethod)一、测定原理BUN(bloodureanitrogen)测试盒-脲酶法尿酸是人体内蛋白代谢的主要终产物,它构成了血液中绝大部分的非蛋白氮。血中尿素氮来源于肝脏,通过肾脏排出体外。肾脏衰竭、肾炎、泌尿道梗阻等可使血液尿素氮含量升高。ALT/GPT(glutamic-pyruvictransaminase/glutamic-pyruvicaminotransferase)测试盒-赖氏法Glutamic谷氨酸的pyruvic丙酮酸transaminase转氨酶Alanine丙氨酸aminotransferase转氨酶丙氨酸丙酮酸ALT/GPT在37℃,作用于丙氨酸及α-酮戊二酸组成的底物,生成丙酮酸及谷氨酸。反应30min后(固定时间)加入2,4-二硝基苯肼(DNPH)盐酸溶液,即中止反应,同时2,4-二硝基苯肼(DNPH)与酮酸中羰基加成,生成丙酮酸苯腙。苯腙在碱性条件下呈红棕色,于505nm比读吸光度并计算酶活力。AST/GOT(aspartatetransaminase/glutamicoxalacetictransaminase)测试盒-Reitman-FrankMethodAspartate天门冬氨酸Glutamic谷氨酸的oxalacetic草酰乙酸的天门冬氨酸—