利用微卫星标记的研究9个地方鸡种的遗传多样性.pdf

利用微卫星标记研究9个地方鸡种的遗传多样性研究生:沈见成导师:王金玉教授陈宽维研究员(扬问大学动物科学与技术学院) 中文摘要利用20个微卫星标记对9个地方鸡种进行遗传多样性分析,根据等位基因频率、多态信息含量、杂合度、有效等位基因数、遗传距离和聚类结果,: 9个地方鸡种在20个微卫星座位中共检测到168个等位基因,其中43个等位基因为所有品种共有,19个稀有等位基因为品种所特有。在位点中,不同座位上的等位基因数在6~12之间,。在品种中,品种拥有的等位基因数在85~121,。 (~), ,-。 (~),,。 (~),,。 9个地方鸡种被聚为4类,1类:淮南三黄鸡、淮北麻鸡、桃源鸡和大骨鸡聚为一类;2 类:寿光鸡、溧阳鸡和狼山鸡聚为一类;3类;丝羽乌骨鸡独自为一类:4类:漳州斗鸡独自为一类。本研究结果可为我田地方鸡种的种质特性研究提供基础致据。为地方鸡种资源的合理保护和利用提供科学依据. 关键词:徽卫星:遗传多样性;地方鸡种 Study on ic Diversity of9Indigenous Chicken Breeds Using MicrosatelliteMarker :ShenJian—cheng Advisor: Jing—yu -wei (Animal Science&Technology College,Yangzhou University) The ic diversiVy of 9 indigenous chicken breeds inChina was surveyed by 20 means oftheallelefrequencies,polymorphism information content(PlCX heterozygosity(H),effective number ofalleles,ic distanceandresultsofdendrogram,ic variabilityandpopulation structure inindigenous chicken breeds were resultswere as foIlows: Forthe 20microsatellite lociexamined,the totalnumber ofalleleswas 168 in9breeds,43 alleles were owned inevery breed and19艄alleles number ofallelesper locus ranged from 6 themeannumber ofallelesper locus was ofallelesper breedranged from 85 to121,and themeannumber ofallelesper breed was . The Mean PIC of20 microsatelliteloci in9indigenous chicken (~ ).Mean PIC ofMCW32 was thehi【ghest()and thatofADLl23 was thelowest m,4294)in number ofMean effectivealleles ineverylocusranged . Mean heterozygosivy of 9 indigenous chickenbreeds in 20 microsatellite loci was (~).Mean heter