角蝇生活习性及斯氏副柔线虫感染情况的调查的分析.pdf

摘要 Y2600505 斯氏副柔线虫(Parabronema skrjabini)是一种寄生在骆驼皱胃的寄生性线虫, 其中间宿主为截脉角蝇(Haematobia titiUans)和西方角蝇(Haematobia irritans)。本文旨在对我国骆驼主要养殖地区角蝇的生活习性以及角蝇感染斯氏副柔线虫的情况进行调查研究。通过对不同地区、不同时间、不同气候条件下角蝇的生活习性进行观察;并在观察的同时采集角蝇,利用生物学剖解方法和分子生物学技术对角蝇体内斯氏副柔线虫幼虫进行观察鉴定,统计角蝇体内斯氏副柔线虫的感染率和感染强度:进而结合采样地点的气候条件对角蝇的分布状况、雌雄比例、斯氏副柔线虫感染情况的影响进行对比分析;同时对角蝇体表及其幼虫生活的粪便环境中的相关昆虫进行鉴定,探讨影响角蝇生活习性的相关因素。结果表明:角蝇幼虫生长发育依赖于粪便,成蝇群居于骆驼等其他反刍动物体表,以吸食宿主血液为生,驱之则散,片刻即聚,受制于气候变化,嗜温厌冷,趋光避风。%,%,%;截脉角蝇为 %,%;%,%。最大感染强度为73条, 。所调查地区均以截脉角蝇为优势种群,其中巴彦淖尔市占 %,%。上述地区角蝇体内斯氏副柔线虫的感染率与截脉角蝇数量呈正比关系。角蝇的雌雄比例与采样地点的月平均气温呈反比关系。角蝇体表寄生的巨螯螨(Macrocheles)和角蝇幼虫所生活的粪便中的胸廓鼓翅蝇(Sepsis cynipsea)幼虫都可能对角蝇的生长发育造成一定影响。本论文的研究结果为弄清角蝇生活习性及其体内斯氏副柔线虫的感染情况,进而研究骆驼斯氏副柔线虫病的防控措施奠定了重要基础。关键诃:角蝇:生活习性:斯氏副柔线虫:感染率:感染强度 Investigation ofHorn FliesHabits andtheir Infectionof Parabronema S奶abini Abstract Par口6,.o,2emaskrjabini,whose intermediate host isHaematobia titillans and HaP聊口tobia irritans,is akindofparasitic nematodes incamel study was carried out toinvestigate theliving habits ofhorn fliesand infection situation of ******@rQb怕nemn skrjabini inmain camel breeding area in our living habitsof hom fliesindifferent areas,different time,and different climate were observated; MeanWhile,Ⅱle hom flies which nuisance camel were collected and Parabronema sbldb讯i larvae inthehornflybody were identified by biological analysis technology and molecular biology techniques;The infection rate and infection intensity of P口,-口6,.o玎e打l订skrjabini inthehom flies was counted;bined with theclimate condition ofsampling locations,it was analyzed thatthehorn fliesdistribution,the proportion ofmales and female Parabronema skrjabini and the infection situation of ParabroFiem口skrjabini;At thesame time,the insects on or around thehorn flies were identifled toinvestigate theenvironmental andbiological factors which impact horn flies’ results showed that:horn fliesdevelopment plete metam