成纤维细胞生长因子在早期人胚胎组织的表达.doc

成纤维细胞生长因子在早期人胚胎组织的表达
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作者:杨拯廖昌军陈建敏荣成刘静陈国庆张晓
【摘要】目的研究成纤维细胞生长因子(Fibroblast Growth Factor,B-FGF)在人胚早期发育的表达。方法用胚胎龄51天的人胚胎行免疫细胞化学ABC法染色。结果胚胎早期发育第51天,在肝、上下肢芽和表皮等组织器官中观察到有B-FGF免疫阳性反应存在,不同器官其免疫阳性反应的强弱和表达情况有差异。结论在人胚胎发育过程中,B-FGF在肝、上肢芽和表皮等组织器官中分布,提示B-FGF对肝、上肢芽和表皮等组织的增殖起作用。
【关键词】 B-FGF;胚胎;增殖
Abstract:Objective To investigate the expression and the distribution of the fibroblast growth factor during the early development of human The human embryo in 51 days and immunocytochemistry ABC technique with anti-B-FGF antibody were B-FGF immuno-positive substance were observed in the cytoplasm of liver,anterior and posterior limb bud and were differential expression on B-FGF immuno-positive substance in different cells and The expression of B-FGF immuno-positive substance in the liver,limb bud and epidermis might suggest that B-FGF plays an important role in promoting and maintaining the biological function in the development of human embryo.
Key words:B-FGF;human embryo;development
成纤维细胞生长因子(Fibroblast Growth Factor-β,B-FGF)在体内具有广泛的生物学意义,对胚胎发育起着十分重要的作用[1]。为此国外许多学者对胚胎、成人和动物用不同方法对B-FGF的含量,蛋白表达和mRNA进行定位研究[2],但对人类胚胎多种组织的B-FGF的免疫组化研究较少。为了探明B-FGF在胚胎组织的分布情况和可能起到的作用,本实验用免疫组化方法对人胚胎B-FGF的分布进行研究。
1 材料和方法
材料
收集早期人胚胎51天标本3例,用4%多聚甲醛固定后制作8μm厚的石蜡切片。B-FGF抗体(Sigma公司产品),ABC药盒(Vector公司产品)。