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干细胞诱导分化为肝细胞鉴定方法.pdf


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中国组织工程研究与临床康复第 15 卷第 14 期 2011 – 04 – 02 出版 Journal of Clinical Rehab ilitative Tiss ue Engineering Research April 2, 2011 , ISSN 1673-8225 CN 21-1539/R CODEN: ZLKHAH 2645 干细胞诱导分化为肝细胞的鉴定方法★李德强,王人颢 Identification of hepatocytes differentiated from stem cells Li De-qiang, Wang Ren-hao Abstract BACKGROUND: Various methods can induce stem cells differentiate into hepatocytes, so we need a set of convincing method to test. OBJECTIVE: To summarize the identification advances in the methods for testing stem cells differentiate into hepatocytes. METHODS: puter-based search was conducted in Foreign Medical Journal Full-Test Service and Pubmed for English articles about isolation, cultivation and induction of stem cells published between January 1985 and June 2010 with the key wor ds of “stem cells, induction, differentiation, hepatocytes, identification”. Meanwhile, Chinese Journal Full-text Database was undertaken to identify relevant articles published between January 2000 and June 2010. A total of 43 articles were collected. RESULTS AND CONCLUSION: Stem cells are characterized by plasticity. Stimulating factors in vitro and special microenvironment in vivo could induce stem cells differentiate into hepatocyte precursors and mature hepatocytes. Stem cells ca n provide a rich source of liver cells and provi

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