红花注射液抗休克的血液动力学研究.doc

红花注射液抗休克的血液动力学研究.doc红花注射液抗休克的血液动力学研究
作者：狄柯坪，周雪峰，常立功
【摘要】 目的观察红花注射液(CTL)对休克家兔肠系膜微
血管血液流变学的影响，为探讨红花活血化瘀的作用机理提供实验依 据。方法采用股动脉放血法复制家兔休克动物模型，把21只健康雄性 大耳白家兔随机分为正常组、生理盐水对照组(NS)和CTL治疗组， 利用显微录像技术观察休克发牛后肠系膜微血管内血流速度、血流量 和血管口径的改变并定量测定红花对其造成的影响。结果休克发生后 肠系膜微血管中的血流速度减慢、血液灌流量减少，CTL可使肠系膜 微血管中的血流速度加快、血液灌流量增多，在整个实验过程中微血 管口径改变不明显。结论CTL可以增快休克时肠系膜微血管中的血流 速度、增加血液灌流量，具有抗休克的作用，但其作用的发挥不是通 过改变血管口径得以实现的。
【关键词】红花肠系膜活体休克
Abstract： ObjectiveTo observe the antishock effect of Carthamus tinctorius linne (CTL)on blood rheology in order to explore the mechanism・ MethodsAlbino rabbits weighting 2. 0〜 were anesthetized with urethane ( 0. 75g/kg ) and alpha-chloralose ( 30mg/kg ) intravenously. The rabbi ts were ventilated with room air via tracheotomy tube
・ The arterial pressure was monitored with a pressure transducer attached to a polyethylene cannula in the right common carotid artery by four channel polygraph, the intestinal loop was mounted on the stage of an inverted microscope and bathed in balanced Kreb-Ringer solution maintained at 37. 5 °C ・ The inside diameter, blood veloci ty and blood volume of arterioles were measured before and after shock. Changes of BP and microcirculation were observed during the whole experiment・ CTL (200mg/ml/kg) was administered via a cannula in the 1 eft carotid artery after shock. Resul tsThe velocity and blood volume of arterioles were decreased after shock, CTL could increase bo