蝮蛇毒素R3晶型0.28nm分辨率结构.pdf

2001年第 59卷 第 3 ~ 446 化学学报 ACTA CHIMIG~SINICA Ⅵ No 3. 442~ 446 nln分辨率结构 唐亮a 周元聪 林政炯a ( 中国科学院生物物理研究所生物大分子国家实验室北京 100101) ( 中国科学院上海生物化学研究所上海 2C0031) 摘要蝮蛇毒素(a ds d0咖血) 培养出了该毒素新晶型( 3) 28 后模型晶体学R因子降到0 23, 3晶型与其它晶型进行了结构比较结果表明,蝮蛇毒 素分子界面识别部位的一个独特疏水区在各种晶型晶体堆积中均起主要作用,对该疏水区可能的生物学功能进行 了讨论. 关键词蝮蛇毒素,神经毒素,磷脂酶A2,x射线衍射,晶体结构,疏永作用 Structure of A gkistrodotoxin in R 3 C rystal F orm at BlIn R esolution gnNc Ij an ZEOU Yuan—Cor HN Zheng-Ji on~ (。Nat/ona/LabotvaoD "of Bid ogi~ M twrom olecules,Insutute of Bi ~oph) ics ,The c地螂 e Aea& m y D厂& r恻 t& ,100101) ( s 口 Inst aitute 绷 .The Academ y of & 础 ,Sh ~gho2,2C0031) c A l~ rm 't A strodotoxin is a phospholipo se .42 一 type presy“aptic neurotoxin from Agk/strodon ha/ys pallas. T he neurotoxln crystallizes in a new ~ stal form , R 3 , under basic conditions. T he crystal slm cture has been determ ined at 0 .28 m resoluti on by the m olecu lar replacem ent tec hnique and refi ned to a cw stallographic R factor of 0 .23 and reasonab le stereochem istry . The cry stal structure of agkistm dotox in in R 3 fo rm pared w ith those of the other two crystal form s . T he extensive intem tolecular hydm phob ic interactions occurring at th e intedacial recogn ition site of each m olecule play an im portant ro