《全反式维甲酸对k562细胞的诱导分化作用》.pdf

万方数据
一；一船爰盛嚣耋毒黼涮dlncnt()28dto奚靖准AfflIatltwest蕊鹳妒O11al㈣skelemiclepneerent!mous5K562ll住懊臠1ucby-R{F“痌㈣协舯骳』／flflIntncsfild}ktdWenAbstract()bjjve，痵蝌，鷖盯岬州出of(DJ3s71mre4Hlyf5}li}rIlthatcxprKm)fHllぁ埃一盯酐加8IHnwlyoliERA1lndu心ATR^JktlccK562_fkf全反式维甲酸对细胞的诱导分化作用05)RA(>LIAorkpuent⋯，日睁盰，HLUOY}{7ZH0}gzHDUxc_jYAN(j鑞蛐ǎ驢61m小恤charactstlcsfafler1ncubalionAEtApper1age(Liifhtted琺。盯，嗫趀强㈨鰈緈fHl1RA("2．缸，忙發rft1ylw1IkRAK縲廿誫伽hjpronlednlyelddllre[(hln表晓军．搴清奎却夯#詉红．李丰益．碗密．等华西医科太学驸属苇二医院疲啥妓淖R猐全反式维甲酸对肿辔细胞具有广泛的抑制增殖、促进分化作用。本研究通过体外培养柃测埘细胞是否有诱导分化作用．方法采用联苯胺染色、瑞氏染色、非特异性酯酶染色、硝5ndIA1RAj后，细胞出现诱导分化特征。结果0s)ATRAL)13CD7lfml0ATRAK56215d1RAcDl3288c]7l05)I^K562[20035(I)811]丶蔧全反式维甲酸；白血病细胞；；诱导分化【中图分类号【文献标识码恼卤嗪臸—叭一㈣s11lMIIldrcnsMr(iJ(nMdl(Scc6l0041Chlna(******@163t)，【╦11e綿∞皌心d4and砣。軱娃川鰂飏叫盯緃8retlvdywKktk“盯朗“裕琣瑆一目的基旧唑氯蓝还原试验植煌娜旧ǚ椒ḿ傲魇较赴酰鄄炀“辧／眗疘，均可诱导细胞向粒系分化。培养，／瓵组サ腒赴5fnl139K562，处理组的分化细胞数仍明显高于对照组dCr)138／樵蛭7115E0lL2O9(P<0瑃inductiondifferenajd—transretinoic1rducmnttkF1Autllr]琑，辴8K!LIAo(hn01mAT}{Artmm1)rlferamlnvtigalecylmletry1rrlolI皀。ResId(blnbHi39l(562ed1ce縭【琤H)eddlcef”nHlcyHd0{ind1)Hve2mM{09Cllァ7toldiffdentlaIntoJ(bntnpword5ィ琹137【赿‘ⅰ荆瑂踟“zam2200!．chlla縝CheIgdu
万方数据
撩麓嚣魄孝瞅】5}LRA蕊鹌妒雐蚰rmtpnNteatmj戳轐勰骽nm1thenddiff6a6irducectgpandFtivetransfomationhaSeInunojogyAfterincLbatlonⅣ現籬cD71(Diaclne)clIltured総amibics縫wrightsStatisticalalysis鐃踟the11p1chgesWrights蟝鲥曲一(ATRA)whichagents[SoK562RPMI1640(GIBcO)Ireadd出忙l^mdL。lcntmlincreasedmpared窟nLb