psicheck2质粒说明书.doc

psicheck2质粒说明书.docTechnical Bulletin
siCHECK? Vectors
INSTRUCTIONS FOR USE OF PRODUCTS C8011 AND C8021.
PRINTED IN USA.
Revised 6/09
Part# TB329
Ptomega
Promega
Promega Corporation • 2800 Woods Hollow Road • Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 Phone 608-274-433-CFax 608-277-
Part# TB329 Printed in USA.
Page 2 Revised 6/09
Promega Corporation • 2800 Woods Hollow Road • Madison, WI 53711-5399 USA
Toll Free in USA 800-356-952& Phone 608-274-4330Fax 608-277-
Printed in USA. Part# TB329
Revised 6/09 Page #
Promega
1
Promega Corporation • 2800 Woods Hollow Road • Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526- Phone 608-274-433CFax 608-277-
Printed in USA. Part# TB329
Revised 6/09 Page 1
siCHECK? Vectors
use
All tech ni cal literature is available on the Internet at: Please visit the web site to verify that you are using the most curre nt vers ion of this Tech ni cal Bullet in. Please con tact Promega Tech ni cal Services if you have questi ons on of this system. E-mail: ******@
Description
Product Components and Storage Conditions 2
General Considerations 3
siCHECK? Vector Features 3
How the siCHECK? Vectors Work 4
Sample Experiments Using the siCHECK? Vectors 6
siCHECK? Vector Maps 9
siCHECK? Vector Restriction Enzyme Tables 11
Restriction Enzyme Sites for the psiCHECK?-1 Vector 11
Restriction Enzyme Sites for the psiCHECK?-2 Vector 13
siCHECK? Vector Backbones and Components 15
References. 16
Related Products. 18
1. Description
The psiCHECK?-1 Vectof - d)Cat.# C8011) and psiCHECK?-2 Vector- f) (Cat.# C8021) are designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in expression of a target gene fused to the reporter ge