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MethodsX 3 (2016) 315–319

Contents lists available at ScienceDirect
MethodsX

journal homepage:

A simple and effective heat induced antigen
retrieval method

Vinod .*, Denny Jones, Venkatesha Udupa

Department of Toxicology, Glenmark Research Centre, Navi Mumbai 400709, India

A B S T R A C T

In this paper, we describe an additional step to the standard method of heat induced antigen retrieval to improve

the detection of antibody staining of formalin ﬁxed parafﬁn embedded tissue sections. Direct heating of tissues in

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