水稻抗病相关基因OsDRxocl和OsDRxoc2的克隆和功能分析.pdf

ddIl20 DNA dNTP dpi EB l:I)T人 GFP HR IR Kan LB miRNA mRNA PB PCR lyllGS RNA RNAi SI)S distilledantdeionized water deoxyribonucleic acid 重蒸水脱氧核糖核酸 deoxy曲onucleoside triphosphate 脱氧核糖核苷三磷脂 dayspost‘inoculation ethidiumbromide Escherichiacoli ethylene etraceticacid green fluorescentprotein Necrotichypersensitive response invertedrepeat kanamycin microRNA. messenger RNA phosphate buffer polymerase chainreaction post-transcriptional genesilencing ribonucleicacid RNA interference sodium dodecyl sulfate 接种后天数溴化乙锭大肠杆菌乙二胺四乙酸绿色荧光蛋白过敏性坏死反应反向重复卡那霉素 LB培养基微RNA 信使RNA 磷酸缓冲液聚合酶链式反应转录后基因沉默核糖核酸 I州A干扰十二烷基硫酸钠目录 (mRNA differentialdisplay)???????????..12 ?????????????????????????..12 (subtractivecDNAhybridization)?????????..12 (suppression subtractivehybridization,SSH)??????.13 (DNA microarray)?????????????????????????..13 ??????????????.14 ???????????????????..14 ????????????..14 ?????????????????????????l 4 、形态建成的发育调控?????????????????..1 5 ???????????????????????..1 5 ?????????????????1 5 ????????????????????????..1 6 ??????????????..16 ??????????????????????????16 2材料与方法???????????????????????????????1 8 ????????????????????????????l 8 ??????????????????????????????18 ???????????????????????????????1 8 ,cocl和OsDRxoc2基因序列PCR扩增????????????????.1 9 ????????????????????????????.20 ?????????????????2l ???????????????????????2 ??????????????????????22 ??????????????????????????22 ?????????????????????????23 ????????????????????????23 ???????????????????23 ????????????????????23 ?????????????????23 2.