第 12 卷 第 11 期 食品安全质量检测学报 Vo l. 12 No. 11re methanol, and purified using a mixed cation (MCX) solid-phase extraction column. Gradient elution was performed using 5 mmol/L ammonium acetate solution containing % formic acid (A) and acetonitrile (B) as the mobile phases, and quantitation was performed on UPLC using a diode array UV detector. Results The detection limit and quantitation limit of the method were mg/kg and 2 mg/kg, respectively. The linearity was good within the range of ‒ 5 μg/mL (r=1). The recoveries were %‒% at the 2, 10, and 20 mg/kg levels. The relative standard deviation was less than % (n=5). The actual sample determination results were ‒ mg/kg. Conclusion This method has good accuracy and precision, and can effectively determine the ergometrine maleate content in forage, which lays a foundation for the application of ergometrine maleate to animal husbandry in the future. KEY WORDS: ergometrine maleate; ultra performance liquid chromatography; herbage
*通信作者: 严凤, 硕士, 高级畜牧师, 主要研究方向为兽药残留分析。E-mail: ******@ *Corresponding author: YAN Feng, Master, Senior Engineer, S
