Cat. # 3340
For Research Use
Chaperone Plasmid Set
Product Manual
v201405
Ca, since it is a simple system that can be used to express a wide
variety of proteins. However, expression of protein in E. coli often results in various
problems, such as the formation of inclusion bodies and protease degradation of the
protein. These frequently encountered issues often are a result of improper folding of
the expressed proteins.
Molecular chaperones are involved in protein folding, and numerous studies have
been conducted to elucidate the mechanisms of in vivo protein folding. Takara's Chap-
erone Plasmid Set consists of five different types of chaperone plasmids developed by
HSP Research Institute, Inc. The plasmids are designed to enable efficient expression
of multiple molecular chaperones known to work cooperatively in the protein folding
process. It has been reported that coexpression of a target protein with one of these
chaperone plasmids increases recovery of expressed proteins in the soluble fraction.
Such proteins often form inclusion bodies using conventional methods (Figure 1).
II.Components
1.Plasmid pG-KJE8 : 10 ng/μl
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